ArticleIranian journal of parasitology2013
Morpho-Physiological and Biochemical Criteria of Acanthamoeba spp. Isolated from the Egyptian Aquatic Environment.
Article in Iranian journal of parasitology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- Impact of contact lens hygiene risk factors on the prevalence of contact lens-related keratitis in Alexandria-Egypt.Journal of ophthalmic inflammation and infection · 2024Article
- Epidemiological profile of microbial keratitis in Alexandria-Egypt a 5 years retrospective study.Journal of ophthalmic inflammation and infection · 2023Article
- Free-living amoebae and their relationship to air quality in hospital environments: characterization ofParasitology · 2020Article
- Exotic Tourist Destinations and Transmission of Infections by Swimming Pools and Hot Springs-A Literature Review.International journal of environmental research and public health · 2018Review
- Genotypic, physiological, and biochemical characterization of potentially pathogenic Acanthamoeba isolated from the environment in Cairo, Egypt.Parasitology research · 2016Article
- Morphological characterization of potentially pathogenic thermophilic amoebae isolated from surface water in mashhad, iran.Jundishapur journal of microbiology · 2015Article
- Acanthamoeba species in Swimming Pools of Cairo, Egypt.Iranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe free-living amoebae Acanthamoeba spp., have been recognized as etiologic agents of amoebic encephalitis, keratitis, otitis, lung lesions and other skin infections mainly in immuno-compromised individuals. In this study, morpho-physiological and biochemical characterization of Acanthamoeba strains isolated from the Egyptian aquatic environment were surveyed.
methodsSome Acanthamoeba species were cultivated on non-nutrient agar. Isolated strains of Acanthamoeba were identification based on the morphology of trophic and cyst forms in addition to temperature and osmo-tolerance assays. Biochemical characterization of the isolated amoeba strains was performed using quantitative assay as well as qualitative determination of proteolytic activity in zymograph analysis.
resultsPotentially pathogenic Acanthamoeba species were isolated from all of the examined water sources. Colorimetric assays showed protease activity in the heat-tolerant isolates of Acanthamoeba. All pathogenic isolates of Acanthamoeba exhibited higher protease activity than did the non-pathogenic ones. The zymographic protease assays showed various banding patterns for different strains of Acanthamoeba.
conclusionThe incidence and prevalence of the pathogenic Acanthamoeba species in the aquatic environment using parasitological and biochemical diagnostic tools will provide baseline data against which the risk factors associated with waterborne transmission can be identified.
Indexed as
Identifiers
23914245PMC3724157W2189128354What OpenQuestion holds
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