ArticleIranian journal of parasitology2013
Application of the Reverse Line Blot Assay for the Molecular Detection of Theileria and Babesia sp. in Sheep and Goat Blood Samples from Pakistan.
Article in Iranian journal of parasitology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- Theileria annulata diagnostics: past, present and future.Tropical animal health and production · 2026Review
- Molecular detection and characterization of Anaplasma ovis, Theileria ovis, and Theileria lestoquardi in sheep and goats in Luxor, Egypt.BMC veterinary research · 2024Article
- Microscopic and molecular detection of piroplasms among sheep in Upper Egypt.Frontiers in veterinary science · 2024Article
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- Identification, molecular characterization and risk factors ofAnimal biotechnology · 2023Article
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- First Molecular Identification ofPathogens (Basel, Switzerland) · 2022Article
- Molecular Detection and Differentiation of Different Theileria Species in Naturally Infected Goats Using Nested PCR-RFLP: A First Report from Northern India.Acta parasitologica · 2022Article
- Molecular identification of Theileria species in naturally infected sheep using nested PCR-RFLP.Parasitology research · 2022Article
- Tick-borne haemoparasitic diseases in small ruminants in Pakistan: Current knowledge and future perspectives.Saudi journal of biological sciences · 2022Review
- Identification of Theileria spp. and investigation of hematological profiles of their infections in goats in Hainan Island, China.Parasite (Paris, France) · 2022Article
- Molecular identification of Theileria equi, Babesia caballi, and Rickettsia in adult ticks from North of Xinjiang, China.Veterinary medicine and science · 2021Article
- Infection by haemopathogens and tick infestation of sheep during summer season in Constantine region, Northeast Algeria.Veterinary medicine and science · 2021Article
- Phylogenetic study ofVeterinary world · 2021Article
- Identification ofPeerJ · 2021Article
- Systematic Review of Ticks and Tick-Borne Pathogens of Small Ruminants in Pakistan.Pathogens (Basel, Switzerland) · 2020Review
- An Assessment of the Molecular Diversity of Ticks and Tick-Borne Microorganisms of Small Ruminants in Pakistan.Microorganisms · 2020Article
- The host-specificity ofParasitology · 2020Article
- Bovine ticks harbour a diverse array of microorganisms in Pakistan.Parasites & vectors · 2020Article
- First molecular survey of piroplasm species in cattle from Kyrgyzstan.Parasitology research · 2019Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe present study was designed to detect the presence of tick-borne parasites (Theileria and Babesia spp.) in 196 blood samples collected from apparently healthy sheep and goats from two provinces, Punjab and Khyber Pukhtoon Khwa, in Pakistan.
methodsReverse line blot (RLB) assay was applied for the parasitic detection by the amplification of hypervariable V4 region of the 18S ribosomal RNA (rRNA) gene. A membrane with covalently linked generic and species specific oligonucleotide probes was used for the hybridization of amplified PCR products.
resultsParasites were detected in 16% of the ruminant blood samples under study. Two Theileria species, T. lestoquardi and T. ovis, were identified in samples. 25, of the total 32, infected animals were from Khyber Pukhtoon Khwa.
conclusionSheep were more prone to tick borne haemoprotozans as 81% infected samples were sheep as compared to 19% goats (P > 0.001). Risk factor analysis revealed that male (P = 0.03), animals infested by ticks (P = 0.03) and herd composed of sheep only (P = 0.001) were more infected by blood parasites.
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Identifiers
23914243PMC3724155W2113526406What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.