ArticleJournal of toxicologic pathology2013
Detection of γ-H2AX, a Biomarker for DNA Double-strand Breaks, in Urinary Bladders of N -Butyl- N -(4-Hydroxybutyl)-Nitrosamine-Treated Rats.
Article in Journal of toxicologic pathology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
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- Early detection of urinary bladder carcinogens in rats by immunohistochemistry for γ-H2AX: a review from analyses of 100 chemicals.Journal of toxicologic pathology · 2022Review
- mRNA translation is a therapeutic vulnerability necessary for bladder epithelial transformation.JCI insight · 2021Article
- Expression of stem cell markers as useful complementary factors in the early detection of urinary bladder carcinogens by immunohistochemistry for γ-H2AX.Archives of toxicology · 2021Article
- Short-term detection of gastric genotoxicity using the DNA double-strand break marker γ-H2AX.Journal of toxicologic pathology · 2019Article
- γH2AX is immunohistochemically detectable until 7 days after exposure of N-bis (2-hydroxypropyl) nitrosamine (DHPN) in rat lung carcinogenesis.Journal of toxicologic pathology · 2018Article
- Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote Organs.Nanomaterials (Basel, Switzerland) · 2017Article
- Reduced DNA double-strand break repair capacity and risk of squamous cell carcinoma of the head and neck--A case-control study.DNA repair · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
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Abstract
To evaluate the potential role of DNA repair in bladder carcinogenesis, we performed an immunohistochemical analysis of expression of various DNA repair enzymes and γ-H2AX, a high-sensitivity marker of DNA double-strand breaks, in the urothelium of male F344 rats treated with N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN), a bladder-specific carcinogen. Our results clearly demonstrated that γ-H2AX aggregation was specifically generated in nuclei of bladder epithelial cells of BBN-treated rats, which was not found in untreated controls or mesenchymal cells. γ-H2AX-positive cells were detected not only in hyperplastic and neoplastic areas but also in the normal-like urothelium after BBN treatment. These data indicate that γ-H2AX has potential as a useful biomarker for early detection of genotoxicity in the rat urinary bladder. To the best of our knowledge, this is the first report demonstrating expression of γ-H2AX during bladder carcinogenesis.
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