ArticleEukaryotic cell2013
Candida albicans VMA3 is necessary for V-ATPase assembly and function and contributes to secretion and filamentation.
Article in Eukaryotic cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 46 citations in OpenAlex.
- Synthetic Lethal Therapy Based on Dimorphism for Systemic Infection of Drug-Resistant Candida albicans.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The V-ATPase VJournal of oral microbiology · 2026Article
- Phenolic Compounds Analysis and In Vitro Biological Activities of Aqueous Extract From Leaves ofFood science & nutrition · 2025Article
- V-ATPase contributes to the cariogenicity of Candida albicans- Streptococcus mutans biofilm.NPJ biofilms and microbiomes · 2025Article
- Surface adherence and vacuolar internalization of bacterial pathogens to the Candida spp. cells: Mechanism of persistence and propagation.Journal of advanced research · 2023Review
- A C. albicans TRAPP Complex-Associated Gene Contributes to Cell Wall Integrity, Hyphal and Biofilm Formation, and Tissue Invasion.Microbiology spectrum · 2023Article
- Candida albicansMicrobiology spectrum · 2022Article
- Magnesium impairs Candida albicans immune evasion by reduced hyphal damage, enhanced β-glucan exposure and altered vacuole homeostasis.PloS one · 2022Article
- Phytochemical Screening and In Vitro Antifungal Activity of Selected Medicinal Plants againstAdvances in pharmacological and pharmaceutical sciences · 2022Article
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- Candida albicansmSphere · 2021Article
- Functional connections between cell cycle and proteostasis in the regulation of Candida albicans morphogenesis.Cell reports · 2021Review
- The Importance of Vacuolar Ion Homeostasis and Trafficking in Hyphal Development and Virulence inFrontiers in microbiology · 2021Review
- Protein Homeostasis Networks and the Use of Yeast to Guide Interventions in Alzheimer's Disease.International journal of molecular sciences · 2020Review
- The Role of Secretory Pathways inJournal of fungi (Basel, Switzerland) · 2020Review
- Ammonium transceptors: Novel regulators of fungal development.PLoS pathogens · 2019Article
- Article
- Effects of Disruption of PMC1 in the tfp1∆/∆ Mutant on Calcium Homeostasis, Oxidative and Osmotic Stress Resistance in Candida albicans.Mycopathologia · 2018Article
- Promising Antifungal Targets AgainstFrontiers in cellular and infection microbiology · 2018Review
- Chemical inhibitors of Candida albicans hyphal morphogenesis target endocytosis.Scientific reports · 2017Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The vacuolar membrane ATPase (V-ATPase) is a protein complex that utilizes ATP hydrolysis to drive protons from the cytosol into the vacuolar lumen, acidifying the vacuole and modulating several key cellular response systems in Saccharomyces cerevisiae. To study the contribution of V-ATPase to the biology and virulence attributes of the opportunistic fungal pathogen Candida albicans, we created a conditional mutant in which VMA3 was placed under the control of a tetracycline-regulated promoter (tetR-VMA3 strain). Repression of VMA3 in the tetR-VMA3 strain prevents V-ATPase assembly at the vacuolar membrane and reduces concanamycin A-sensitive ATPase-specific activity and proton transport by more than 90%. Loss of C. albicans V-ATPase activity alkalinizes the vacuolar lumen and has pleiotropic effects, including pH-dependent growth, calcium sensitivity, and cold sensitivity. The tetR-VMA3 strain also displays abnormal vacuolar morphology, indicative of defective vacuolar membrane fission. The tetR-VMA3 strain has impaired aspartyl protease and lipase secretion, as well as attenuated virulence in an in vitro macrophage killing model. Repression of VMA3 suppresses filamentation, and V-ATPase-dependent filamentation defects are not rescued by overexpression of RIM8, MDS3, EFG1, CST20, or UME6, which encode positive regulators of filamentation. Specific chemical inhibition of Vma3p function also results in defective filamentation. These findings suggest either that V-ATPase functions downstream of these transcriptional regulators or that V-ATPase function during filamentation involves independent mechanisms and alternative signaling pathways. Taken together, these data indicate that V-ATPase activity is a fundamental requirement for several key virulence-associated traits in C. albicans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.