ArticleApplied and environmental microbiology2013
Engineered biosynthesis of disaccharide-modified polyene macrolides.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 18 citations in OpenAlex.
- Engineered biosynthesis and characterization of disaccharide-pimaricin.Microbial cell factories · 2025Article
- Effect of Post-Polyketide Synthase Modification Groups on Property and Activity of Polyene Macrolides.Antibiotics (Basel, Switzerland) · 2023Review
- New Glycosylated Polyene Macrolides: Refining the Ore from Genome Mining.Antibiotics (Basel, Switzerland) · 2022Review
- Screening and Purification of Natural Products from Actinomycetes that Induce a "Rounded" Morphological Phenotype in Fission Yeast.Natural products and bioprospecting · 2021Article
- Amphotericin B biosynthesis in Streptomyces nodosus: quantitative analysis of metabolism via LC-MS/MS based metabolomics for rational design.Microbial cell factories · 2020Article
- Post-PKS tailoring steps of a disaccharide-containing polyene NPP in Pseudonocardia autotrophica.PloS one · 2015Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Recent work has uncovered genes for two glycosyltransferases that are thought to catalyze mannosylation of mycosaminyl sugars of polyene macrolides. These two genes are nypY from Pseudonocardia sp. strain P1 and pegA from Actinoplanes caeruleus. Here we analyze these genes by heterologous expression in various strains of Streptomyces nodosus, producer of amphotericins, and in Streptomyces albidoflavus, which produces candicidins. The NypY glycosyltransferase converted amphotericins A and B and 7-oxo-amphotericin B to disaccharide-modified forms in vivo. The enzyme did not act on amphotericin analogs lacking exocyclic carboxyl or mycosamine amino groups. Both NypY and PegA acted on candicidins. This work confirms the functions of these glycosyltransferases and provides insights into their acceptor substrate tolerance. Disaccharide-modified polyenes may have potential as less toxic antibiotics.
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Registered trials
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