ArticleJournal of biomedical optics2013
Tumor-associated macrophages and stromal TNF-α regulate collagen structure in a breast tumor model as visualized by second harmonic generation.
Article in Journal of biomedical optics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Targeting Tumor-Associated Macrophages to Reshape the Immuno-Mechanical Landscape: Molecular Mechanisms and Therapeutic Strategies.International journal of biological sciences · 2026Review
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- QuantifyingBiophotonics discovery · 2024Article
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- Breast cancer microenvironment and obesity: challenges for therapy.Cancer metastasis reviews · 2022Review
- Can't handle the stress? Mechanobiology and disease.Trends in molecular medicine · 2022Review
- TRIPODD: a Novel Fluorescence Imaging Platform for In Situ Quantification of Drug Distribution and Therapeutic Response.Molecular imaging and biology · 2021Article
- Microenvironmental modulation of the developing tumour: an immune-stromal dialogue.Molecular oncology · 2021Review
- Macrophages and Extracellular Matrix in Breast Cancer: Partners in Crime or Protective Allies?Frontiers in oncology · 2021Review
- Pro-tumorigenic functions of macrophages at the primary, invasive and metastatic tumor site.Cancer immunology, immunotherapy : CII · 2020Review
- Second-harmonic generation scattering directionality predicts tumor cell motility in collagen gels.Journal of biomedical optics · 2015Article
- The Use of Second Harmonic Generation to Image the Extracellular Matrix During Tumor Progression.Intravital · 2014Article
- Intima modifier locus 2 controls endothelial cell activation and vascular permeability.Physiological genomics · 2014Article
- Live imaging and gene expression analysis in zebrafish identifies a link between neutrophils and epithelial to mesenchymal transition.PloS one · 2014Article
- The antidepressant desipramine and α2-adrenergic receptor activation promote breast tumor progression in association with altered collagen structure.Cancer prevention research (Philadelphia, Pa.) · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Collagen fibers can be imaged with second harmonic generation (SHG) and are associated with efficient tumor cell locomotion. Preferential locomotion along these fibers correlates with a more aggressively metastatic phenotype, and changes in SHG emission properties accompany changes in metastatic outcome. We therefore attempted to elucidate the cellular and molecular machinery that influences SHG in order to understand how the microstructure of tumor collagen fibers is regulated. By quantifying SHG and immunofluorescence (IF) from tumors grown in mice with and without stromal tumor necrosis factor (TNF)-α and in the presence or absence of tumor-associated macrophages (TAMs), we determined that depletion of TAMs alters tumor collagen fibrillar microstructure as quantified by SHG and IF. Furthermore, we determined that abrogation of TNF-α expression by tumor stromal cells also alters fibrillar microstructure and that subsequent depletion of TAMs has no further effect. In each case, metastatic burden correlated with optical readouts of collagen microstructure. Our results implicate TAMs and stromal TNF-α as regulators of breast tumor collagen microstructure and suggest that this regulation plays a role in tumor metastasis. Furthermore, these results indicate that quantification of SHG represents a useful strategy for evaluating the cells and molecular pathways responsible for manipulating fibrillar collagen in breast tumor models.
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