ArticleAnnals of botany2013
Microsatellite and flow cytometry analysis to help understand the origin of Dioscorea alata polyploids.
Article in Annals of botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Uncovering the genetic diversity of yams (Ecology and evolution · 2021Article
- Genetic control of flowering in greater yam (Dioscorea alata L.).BMC plant biology · 2021Article
- Genome-wide genotyping elucidates the geographical diversification and dispersal of the polyploid and clonally propagated yam (Dioscorea alata).Annals of botany · 2020Article
- Development of a cost-effective single nucleotide polymorphism genotyping array for management of greater yam germplasm collections.Ecology and evolution · 2019Article
- A Target Capture-Based Method to Estimate Ploidy From Herbarium Specimens.Frontiers in plant science · 2019Article
- Genetic diversity and population structure of trifoliate yam (Dioscorea dumetorum Kunth) in Cameroon revealed by genotyping-by-sequencing (GBS).BMC plant biology · 2018Article
- Understanding the genetic diversity and population structure of yam (Dioscorea alata L.) using microsatellite markers.PloS one · 2017Article
- 2n megagametophyte formed via SDR contributes to tetraploidization in polyembryonic 'Nadorcott' tangor crossed by citrus allotetraploids.Plant cell reports · 2014Article
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6 authors.
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Abstract
BACKGROUND AND
aimsDioscorea alata is a polyploid species with a ploidy level ranging from diploid (2n = 2x = 40) to tetraploid (2n = 4x = 80). Ploidy increase is correlated with better agronomic performance. The lack of knowledge about the origin of D. alata spontaneous polyploids (triploids and tetraploids) limits the efficiency of polyploid breeding. The objective of the present study was to use flow cytometry and microsatellite markers to understand the origin of D. alata polyploids.
methodsDifferent progeny generated by intracytotype crosses (2x × 2x) and intercytotype crosses (2x × 4x and 3x × 2x) were analysed in order to understand endosperm incompatibility phenomena and gamete origins via the heterozygosity rate transmitted to progeny.
resultsThis work shows that in a 2x × 2x cross, triploids with viable seeds are obtained only via a phenomenon of diploid female non-gametic reduction. The study of the transmission of heterozygosity made it possible to exclude polyspermy and polyembryony as the mechanisms at the origin of triploids. The fact that no seedlings were obtained by a 3x × 2x cross made it possible to confirm the sterility of triploid females. Flow cytometry analyses carried out on the endosperm of seeds resulting from 2x × 4x crosses revealed endosperm incompatibility phenomena.
conclusionsThe major conclusion is that the polyploids of D. alata would have appeared through the formation of unreduced gametes. The triploid pool would have been built and diversified through the formation of 2n gametes in diploid females as the result of the non-viability of seeds resulting from the formation of 2n sperm and of the non-viability of intercytotype crosses. The tetraploids would have appeared through bilateral sexual polyploidization via the union of two unreduced gametes due to the sterility of triploids.
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