ArticleJournal of virological methods2013
The replication of a mouse adapted SARS-CoV in a mouse cell line stably expressing the murine SARS-CoV receptor mACE2 efficiently induces the expression of proinflammatory cytokines.
Article in Journal of virological methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Comparison of infection and human immune responses of two SARS-CoV-2 strains in a humanized hACE2 NIKO mouse model.Scientific reports · 2023Article
- Cleavage of cFLIP restrains cell death during viral infection and tissue injury and favors tissue repair.Science advances · 2023Article
- Article
- MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity.mBio · 2021Article
- The envelope protein of SARS-CoV-2 increases intra-Golgi pH and forms a cation channel that is regulated by pH.The Journal of physiology · 2021Article
- Beneficial Effect of Statins in COVID-19-Related Outcomes-Brief Report: A National Population-Based Cohort Study.Arteriosclerosis, thrombosis, and vascular biology · 2021Article
- Pulmonary pathology of early-phase COVID-19 pneumonia in a patient with a benign lung lesion.Histopathology · 2020Article
- Advances in the relationship between coronavirus infection and cardiovascular diseases.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2020Review
- SARS-CoV-Encoded Small RNAs Contribute to Infection-Associated Lung Pathology.Cell host & microbe · 2017Article
- Identification of the Mechanisms Causing Reversion to Virulence in an Attenuated SARS-CoV for the Design of a Genetically Stable Vaccine.PLoS pathogens · 2015Article
- The PDZ-binding motif of severe acute respiratory syndrome coronavirus envelope protein is a determinant of viral pathogenesis.PLoS pathogens · 2014Article
- Severe acute respiratory syndrome coronavirus envelope protein ion channel activity promotes virus fitness and pathogenesis.PLoS pathogens · 2014Article
- Inhibition of NF-κB-mediated inflammation in severe acute respiratory syndrome coronavirus-infected mice increases survival.Journal of virology · 2014Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Infection of conventional mice with a mouse adapted (MA15) severe acute respiratory syndrome (SARS) coronavirus (CoV) reproduces many aspects of human SARS such as pathological changes in lung, viremia, neutrophilia, and lethality. However, established mouse cell lines highly susceptible to mouse-adapted SARS-CoV infection are not available. In this work, efficiently transfectable mouse cell lines stably expressing the murine SARS-CoV receptor angiotensin converting enzyme 2 (ACE2) have been generated. These cells yielded high SARS-CoV-MA15 titers and also served as excellent tools for plaque assays. In addition, in these cell lines, SARS-CoV-MA15 induced the expression of proinflammatory cytokines and IFN-β, mimicking what has been observed in experimental animal models infected with SARS-CoV and SARS patients. These cell lines are valuable tools to perform in vitro studies in a mouse cell system that reflects the species used for in vivo studies of SARS-CoV-MA15 pathogenesis.
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