ArticleInsect biochemistry and molecular biology2013
RNAi-based demonstration of direct link between specific odorant receptors and mosquito oviposition behavior.
Article in Insect biochemistry and molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 53 citations in OpenAlex.
- Expanding the sensory horizon: chemosensory receptors beyond traditional olfactory and taste organs in insects.Science China. Life sciences · 2026Review
- UnravelingFrontiers in pharmacology · 2025Review
- RNAi-Mediated Silencing ofInsects · 2024Article
- Odorant receptors for floral- and plant-derived volatiles in the yellow fever mosquito, Aedes aegypti (Diptera: Culicidae).PloS one · 2024Article
- Two odorant receptors regulate 1-octen-3-ol induced oviposition behavior in the oriental fruit fly.Communications biology · 2023Article
- A highly expressed odorant receptor from the yellow fever mosquito, AaegOR11, responds to (+)- and (-)-fenchone and a phenolic repellent.Insect biochemistry and molecular biology · 2022Article
- Mosquito odorant receptor sensitive to natural spatial repellents and inhibitory compounds.Insect biochemistry and molecular biology · 2022Article
- Screening of olfactory genes related to blood-feeding behaviors in Culex pipiens quinquefasciatus and Culex pipiens molestus by transcriptome analysis.PLoS neglected tropical diseases · 2022Article
- Mutagenesis of the orco odorant receptor co-receptor impairs olfactory function in the malaria vector Anopheles coluzzii.Insect biochemistry and molecular biology · 2020Article
- Rat volatiles as an attractant source for the Asian tiger mosquito, Aedes albopictus.Scientific reports · 2020Article
- The olfactory basis of orchid pollination by mosquitoes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- DEET and other repellents are inhibitors of mosquito odorant receptors for oviposition attractants.Insect biochemistry and molecular biology · 2019Article
- Odorant receptors from Culex quinquefasciatus and Aedes aegypti sensitive to floral compounds.Insect biochemistry and molecular biology · 2019Article
- Conspecific and allospecific larval extracts entice mosquitoes to lay eggs and may be used in attract-and-kill control strategy.Scientific reports · 2019Article
- RNA interference-mediated knockdown of 3, 4-dihydroxyphenylacetaldehyde synthase affects larval development and adult survival in the mosquito Aedes aegypti.Parasites & vectors · 2019Article
- Reverse chemical ecology-based approach leading to the accidental discovery of repellents for Rhodnius prolixus, a vector of Chagas diseases refractory to DEET.Insect biochemistry and molecular biology · 2018Article
- Molecules as Biotic Messengers.ACS omega · 2018Review
- Modulation of Host Learning in Aedes aegypti Mosquitoes.Current biology : CB · 2018Article
- Reverse chemical ecology approach for the identification of an oviposition attractant forProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The Southern house mosquito, Culex quinquefasciatus--a vector of West Nile virus--is equipped with 130 odorant receptors (ORs), which enable young females to locate plants and blood-meal sources and older females to find suitable sites for oviposition. In our attempts to de-orphanize ORs expressed in female antennae, we identified CquiOR37 and CquiOR99, which were narrowly tuned to two phenolic compounds, 4-methylphenol and 4-ethylphenol. When tested in the Xenopus oocyte recording system the observed EC50s for 4-methylphenol and 4-ethylphenol were 6.4 and 18.2 μM for CquiOR37 and 14.4 and 0.74 μM for CquiOR99 (goodness of fit, R² = 0.88-0.99), respectively. Indoor behavioral assays demonstrated that gravid female mosquitoes laid significantly more eggs in water trays spiked with these compounds than in control water trays. Field studies with gravid traps corroborated that 4-ethylphenol is active in a wide range of doses from 0.1 to 10 μg/l, as required for practical applications. A dsRNA construct based on the two genes, CquiOR37/99-dsRNA was stable in pupa hemolymph for up to 3 h. Pupae injected with CquiOR37/99-dsRNA, β-galactosidase-dsRNA or water had more than 40% survival rate at the peak of oviposition (day-9). qPCR analysis showed individual variation, but significant mean reduction in CquiOR37 and CquiOR99 transcript levels in CquiOR37/99-dsRNA-treated mosquitoes. Water-injected females and those treated with the control gene laid significantly more eggs in trays containing 4-ethylphenol than in water trays, whereas CquiOR37/99-dsRNA-treated mosquitoes laid normal number of eggs, but could not discriminate treatment from control. This study linked for the first time specific receptors for 4-ethylphenol with increased oviposition in the important vector Cx. quinquefasciatus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.