ArticleThe European journal of neuroscience2013
GABA(A) receptors can initiate the formation of functional inhibitory GABAergic synapses.
Article in The European journal of neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 38 citations in OpenAlex.
- Neurobiological subtypes of adolescent depression: a multimodal integration of morphometric similarity network and spatial transcriptomics.Molecular psychiatry · 2025Article
- Editorial: Cellular and molecular mechanisms that govern assembly, plasticity, and function of GABAergic inhibitory circuits in the mammalian brain.Frontiers in cellular neuroscience · 2025Article
- GABAFrontiers in cellular neuroscience · 2024Article
- Molecular changes underlying decay of sensory responses and enhanced seizure propensity in peritumoral neurons.Neuro-oncology · 2023Article
- Anti-seizure mechanisms of midazolam and valproate at the β2(L51M) variant of the GABANeuropharmacology · 2022Article
- Augmenting neurogenesis rescues memory impairments in Alzheimer's disease by restoring the memory-storing neurons.The Journal of experimental medicine · 2022Article
- Role of Omics in Migraine Research and Management: A Narrative Review.Molecular neurobiology · 2022Review
- Induction of synapse formation by de novo neurotransmitter synthesis.Nature communications · 2022Article
- Early Developmental PMCA2b Expression Protects From Ketamine-Induced Apoptosis and GABA Impairments in Differentiating Hippocampal Progenitor Cells.Frontiers in cellular neuroscience · 2022Article
- Identification of a Signature for Predicting Prognosis and Immunotherapy Response in Patients with Glioma.Journal of oncology · 2022Article
- Gabaergic Interneurons in Early Brain Development: Conducting and Orchestrated by Cortical Network Activity.Frontiers in molecular neuroscience · 2021Article
- Synapse and Receptor Alterations in Two Different S100B-Induced Glaucoma-Like Models.International journal of molecular sciences · 2020Article
- Selective Modulation of α5 GABAFrontiers in cellular neuroscience · 2020Article
- Effects of GABAFrontiers in molecular neuroscience · 2020Article
- Adeno-associated viral overexpression of neuroligin 2 in the mouse hippocampus enhances GABAergic synapses and impairs hippocampal-dependent behaviors.Behavioural brain research · 2019Article
- Genetic Deletion of GABAFrontiers in cellular neuroscience · 2019Article
- An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins.Journal of visualized experiments : JoVE · 2018Article
- GABA type a receptor trafficking and the architecture of synaptic inhibition.Developmental neurobiology · 2018Review
- SAHA (Vorinostat) Corrects Inhibitory Synaptic Deficits Caused by Missense Epilepsy Mutations to the GABAFrontiers in molecular neuroscience · 2018Article
- An Essential Role for the Tetraspanin LHFPL4 in the Cell-Type-Specific Targeting and Clustering of Synaptic GABACell reports · 2017Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
The mechanisms that underlie the selection of an inhibitory GABAergic axon's postsynaptic targets and the formation of the first contacts are currently unknown. To determine whether expression of GABAA receptors (GABAA Rs) themselves--the essential functional postsynaptic components of GABAergic synapses--can be sufficient to initiate formation of synaptic contacts, a novel co-culture system was devised. In this system, the presynaptic GABAergic axons originated from embryonic rat basal ganglia medium spiny neurones, whereas their most prevalent postsynaptic targets, i.e., α1/β2/γ2-GABAA Rs, were expressed constitutively in a stably transfected human embryonic kidney 293 (HEK293) cell line. The first synapse-like contacts in these co-cultures were detected by colocalization of presynaptic and postsynaptic markers within 2 h. The number of contacts reached a plateau at 24 h. These contacts were stable, as assessed by live cell imaging; they were active, as determined by uptake of a fluorescently labelled synaptotagmin vesicle-luminal domain-specific antibody; and they supported spontaneous and action potential-driven postsynaptic GABAergic currents. Ultrastructural analysis confirmed the presence of characteristics typical of active synapses. Synapse formation was not observed with control or N-methyl-d-aspartate receptor-expressing HEK293 cells. A prominent increase in synapse formation and strength was observed when neuroligin-2 was co-expressed with GABAA Rs, suggesting a cooperative relationship between these proteins. Thus, in addition to fulfilling an essential functional role, postsynaptic GABAA Rs can promote the adhesion of inhibitory axons and the development of functional synapses.
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