ArticleThe EMBO journal1990
Constitutive overexpression of the contact site A glycoprotein enables growth-phase cells of Dictyostelium discoideum to aggregate.
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Generation of Single-Cell Transcript Variability by Repression.Current biology : CB · 2017Article
- The Dictyostelium discoideum GPHR ortholog is an endoplasmic reticulum and Golgi protein with roles during development.Eukaryotic cell · 2015Article
- The centrosomal component CEP161 of Dictyostelium discoideum interacts with the Hippo signaling pathway.Cell cycle (Georgetown, Tex.) · 2015Article
- Article
- Ectopic expression of cyclase associated protein CAP restores the streaming and aggregation defects of adenylyl cyclase a deficient Dictyostelium discoideum cells.BMC developmental biology · 2012Article
- The cyclase-associated protein CAP as regulator of cell polarity and cAMP signaling in Dictyostelium.Molecular biology of the cell · 2004Article
- Villidin, a novel WD-repeat and villin-related protein from Dictyostelium, is associated with membranes and the cytoskeleton.Molecular biology of the cell · 2003Article
- Recruitment of cortexillin into the cleavage furrow is controlled by Rac1 and IQGAP-related proteins.The EMBO journal · 2001Article
- Detection of subtle phenotypes: the case of the cell adhesion molecule csA in Dictyostelium.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Analysis of gene function in Dictyostelium.Experientia · 1995Review
- Cell adhesion in the life cycle of Dictyostelium.Experientia · 1995Review
- Replacement of the phospholipid-anchor in the contact site A glycoprotein of D. discoideum by a transmembrane region does not impede cell adhesion but reduces residence time on the cell surface.The Journal of cell biology · 1994Article
- Actin binding to lipid-inserted alpha-actinin.Biophysical journal · 1993Article
- Transcriptional regulation by folate: inducible gene expression in Dictyostelium transformants during growth and early development.Nucleic acids research · 1992Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The contact site A (csA) glycoprotein is a developmentally regulated cell adhesion molecule which mediates EDTA-stable cell contacts during the aggregation stage of Dictyostelium discoideum. A transformation vector was constructed which allows overexpression of the csA protein during the growth phase. In that stage the csA protein is normally not expressed; in the transformants it was transported to the cell surface and carried all modifications investigated, including a phospholipid anchor and two types of oligosaccharide chain. csA expression enabled the normal non-aggregative growth-phase cells to form EDTA-stable contacts in suspension and to assemble into three-dimensional aggregates when moving on a substratum. After prolonged cultivation of csA overexpressing transformants in nutrient medium the developmental program was found to be turned on, as it normally occurs only in starving cells. During later development of transformed cells, the csA glycoprotein remained present on the cell surface, while it is down-regulated in the wild type. It was detected in both the prestalk and prespore regions of the multicellular slugs made from transformed cells.
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