ArticleBioconjugate chemistry2013
Labeling primary amine groups in peptides and proteins with N-hydroxysuccinimidyl ester modified Fe3O4@SiO2 nanoparticles containing cleavable disulfide-bond linkers.
Article in Bioconjugate chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
- Folate-Functionalized Albumin-Containing Systems: Non-Covalent vs. Covalent Binding of Folic Acid.Pharmaceutics · 2025Review
- Cross-Linking Mass Spectrometry to Capture Protein Network Dynamics of Cell Membranome.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Site-Specific Immobilization Boosts the Performance of a Galectin-1 Biosensor.Bioconjugate chemistry · 2024Article
- Advancing cell surface modification in mammalian cells with synthetic molecules.Chemical science · 2023Review
- DNA Modifications Enabling Proximity Biotinylation.Bioconjugate chemistry · 2023Article
- Design of experiment (DoE)-drivenJournal of extracellular vesicles · 2020Article
- Resin and Magnetic Nanoparticle-Based Free Radical Probes for Glycan Capture, Isolation, and Structural Characterization.Analytical chemistry · 2019Article
- Susceptibility of microbial cells to the modified PIPJournal of nanobiotechnology · 2019Article
- Harnessing Biology to Deliver Therapeutic and Imaging Entities via Cell-Based Methods.Chemistry (Weinheim an der Bergstrasse, Germany) · 2018Review
- Thiodigalactoside-Bovine Serum Albumin Conjugates as High-Potency Inhibitors of Galectin-3: An Outstanding Example of Multivalent Presentation of Small Molecule Inhibitors.Bioconjugate chemistry · 2018Article
- ColorimetricTheranostics · 2018Article
- In Vitro/In Vivo Toxicity Evaluation and Quantification of Iron Oxide Nanoparticles.International journal of molecular sciences · 2015Review
- Cleavable ester linked magnetic nanoparticles for labeling of solvent exposed primary amine groups of peptides/proteins.Data in brief · 2015Article
- Supramolecular tailoring of protein-nanoparticle interactions using cucurbituril mediators.Chemical communications (Cambridge, England) · 2014Article
- A Comparison of Iron Oxide Particles and Silica Particles for Tracking Organ Recellularization.Molecular imagingArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The surface of superparamagnetic silica coated iron oxide (Fe3O4@SiO2) nanoparticles was functionalized with a disulfide bond linked N-hydroxysuccinimidyl (NHS) ester group in order to develop a method for labeling primary amines in peptides/proteins. The nanoparticle labeled proteins/peptides formed after NHS ester reaction with the primary amine groups were isolated using a magnet without any additional purification step. Nanoparticle moieties conjugated to peptides/proteins were then trimmed by cleavage at the disulfide linker with a reducing agent. The labeled peptides were analyzed by LC-MS/MS to determine their sequences and the sites of NHS ester labeling. This novel approach allowed characterization of lysine residues on the solvent accessible surface of native bovine serum albumin. Low cost, rapid magnetic separation, and specificity toward primary amine groups make NHS ester coated Fe3O4@SiO2 nanoparticles a potential labeling probe to study proteins on living cell surfaces.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.