ArticleZebrafish2013
Development of an in vitro cell system from zebrafish suitable to study bone cell differentiation and extracellular matrix mineralization.
Article in Zebrafish, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Primary Culture and Characterization of a Crucian Carp (Animals : an open access journal from MDPI · 2026Article
- Isolation and Culture of Osteoblasts.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Skeletal Morphogenesis and Anomalies in Gilthead Seabream: A Comprehensive Review.International journal of molecular sciences · 2023Review
- Effect of Early Peptide Diets on Zebrafish Skeletal Development.Biomolecules · 2023Article
- Screening of Mineralogenic and Osteogenic Compounds in Zebrafish-Tools to Improve Assay Throughput and Data Accuracy.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Reversal of Doxorubicin-Induced Bone Loss and Mineralization by Supplementation of Resveratrol and MitoTEMPO in the Early Development ofNutrients · 2022Article
- Cells Isolated from Regenerating Caudal Fin of Sparus aurata Can Differentiate into Distinct Bone Cell Lineages.Marine biotechnology (New York, N.Y.) · 2020Article
- Age, but not short-term intensive swimming, affects chondrocyte turnover in zebrafish vertebral cartilage.PeerJ · 2018Article
- Comparative analysis of zebrafish bone morphogenetic proteins 2, 4 and 16: molecular and evolutionary perspectives.Cellular and molecular life sciences : CMLS · 2016Article
- Adipogenic Gene Expression in Gilthead Sea Bream Mesenchymal Stem Cells from Different Origin.Frontiers in endocrinology · 2016Article
- Review
- Zebrafish vitamin K epoxide reductases: expression in vivo, along extracellular matrix mineralization and under phylloquinone and warfarin in vitro exposure.Fish physiology and biochemistry · 2015Article
- Cellular and molecular characterization of a novel primary osteoblast culture from the vertebrate model organism Xenopus tropicalis.Histochemistry and cell biology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanisms of bone formation and skeletal development have been successfully investigated in zebrafish using a variety of in vivo approaches, but in vitro studies have been hindered due to a lack of homologous cell lines capable of producing an extracellular matrix (ECM) suitable for mineral deposition. Here we describe the development and characterization of a new cell line termed ZFB1, derived from zebrafish calcified tissues. ZFB1 cells have an epithelium-like phenotype, grow at 28°C in a regular L-15 medium supplemented with 15% of fetal bovine serum, and are maintained and manipulated using standard methods (e.g., trypsinization, cryopreservation, and transfection). They can therefore be propagated and maintained easily in most cell culture facilities. ZFB1 cells show aneuploidy with 2n=78 chromosomes, indicative of cell transformation. Furthermore, because DNA can be efficiently delivered into their intracellular space by nucleofection, ZFB1 cells are suitable for gene targeting approaches and for assessing gene promoter activity. ZFB1 cells can also differentiate toward osteoblast or chondroblast lineages, as demonstrated by expression of osteoblast- and chondrocyte-specific markers, they exhibit an alkaline phosphatase activity, a marker of bone formation in vivo, and they can mineralize their ECM. Therefore, they represent a valuable zebrafish-derived in vitro system for investigating bone cell differentiation and extracellular matrix mineralization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.