ArticlePlanta2013
The rice OsLTP6 gene promoter directs anther-specific expression by a combination of positive and negative regulatory elements.
Article in Planta, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- An epi-allele of SMS causes Sanming dominant genic male sterility in rice.Science China. Life sciences · 2023Article
- A bifunctional selectable marker for wheat transformation contributes to the characterization of male-sterile phenotype induced by a synthetic Ms2 gene.Plant cell reports · 2023Article
- A Systematic Investigation of Lipid Transfer Proteins Involved in Male Fertility and Other Biological Processes in Maize.International journal of molecular sciences · 2023Article
- Fruit preferential activity of the tomato RIP1 gene promoter in transgenic tomato and Arabidopsis.Molecular genetics and genomics : MGG · 2017Article
- Article
- Application of rice microspore-preferred promoters to manipulate early pollen development in Arabidopsis: a heterologous system.Plant reproduction · 2016Article
- Review
- Genome-wide identification and analysis of rice genes preferentially expressed in pollen at an early developmental stage.Plant molecular biology · 2016Article
- Identification of cis-elements and evaluation of upstream regulatory region of a rice anther-specific gene, OSIPP3, conferring pollen-specific expression in Oryza sativa (L.) ssp. indica.Plant reproduction · 2015Article
- Identification and expression analysis of BoMF25, a novel polygalacturonase gene involved in pollen development of Brassica oleracea.Plant reproduction · 2015Article
- Tissue-specific expression of a soybean hypersensitive-induced response (HIR) protein gene promoter.Plant molecular biology · 2015Article
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5 authors.
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Abstract
Characterization of tissue-specific plant gene promoters will benefit genetic improvement in crops. Here, we isolated a novel rice anther-specific plant lipid transfer protein (OsLTP6) gene through high through-put expressional profiling. The promoter of OsLTP6 was introduced to the upstream of the uidA gene, which encodes β-glucuronidase (GUS), and transformed into rice plants for functional analysis. Histochemical and fluorometric GUS assay showed that GUS was specifically expressed in the anthers and pollens in OsLTP6 promoter::uidA transgenic plants. Transverse section of the rice anther further indicated that the OsLTP6 promoter directed the reporter gene specifically expressed in anther tapetum. To identify regulatory elements within OsLTP6 promoter region, four progressive deletions of the OsLTP6 promoter were constructed. The results indicated that the OsLTP6 promoter achieved anther-specific expression through a combination of positive and negative regulatory elements. A 26-bp motif upstream of TATA box was a key transcriptional activator for OsLTP6 gene. CAAT box and GTGA box were the putative motifs to increase the transcription level to full expression. Two negative regulatory elements were also found in two distinct regions within this promoter. They repressed the expression in leaf and stem, respectively. These results revealed the regulating complexity of anther-specific expression.
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