ArticleCell cycle (Georgetown, Tex.)2013
Automated tracking of mitotic spindle pole positions shows that LGN is required for spindle rotation but not orientation maintenance.
Article in Cell cycle (Georgetown, Tex.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Deep learning techniques and mathematical modeling allow 3D analysis of mitotic spindle dynamics.The Journal of cell biology · 2023Article
- Counteraction between Astrin-PP1 and Cyclin-B-CDK1 pathways protects chromosome-microtubule attachments independent of biorientation.Nature communications · 2021Article
- Multinucleation associated DNA damage blocks proliferation in p53-compromised cells.Communications biology · 2021Article
- Article
- MARK2/Par1b kinase present at centrosomes and retraction fibres corrects spindle off-centring induced by actin disassembly.Open biology · 2019Article
- Quantitative analysis of sensitivity to a Wnt3a gradient in determination of the pole-to-pole axis of mitotic cells by using a microfluidic device.FEBS open bio · 2018Article
- Spindle rotation in human cells is reliant on a MARK2-mediated equatorial spindle-centering mechanism.The Journal of cell biology · 2018Article
- Microtubule end tethering of a processive kinesin-8 motor Kif18b is required for spindle positioning.The Journal of cell biology · 2018Article
- Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells.Nature communications · 2017Article
- Automated mitotic spindle tracking suggests a link between spindle dynamics, spindle orientation, and anaphase onset in epithelial cells.Molecular biology of the cell · 2017Article
- Vitamin D3 suppresses morphological evolution of the cribriform cancerous phenotype.Oncotarget · 2016Article
- Modeling of Noisy Spindle Dynamics Reveals Separable Contributions to Achieving Correct Orientation.Biophysical journal · 2015Article
- XI-006 induces potent p53-independent apoptosis in Ewing sarcoma.Scientific reports · 2015Article
- A proteomic study of mitotic phase-specific interactors of EB1 reveals a role for SXIP-mediated protein interactions in anaphase onset.Biology open · 2015Article
- A Semi-Supervised Approach for Refining Transcriptional Signatures of Drug Response and Repositioning Predictions.PloS one · 2015Article
- TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes.Open biology · 2014Article
- Need for multi-scale systems to identify spindle orientation regulators relevant to tissue disorganization in solid cancers.Frontiers in physiology · 2014Article
- Astral microtubules control redistribution of dynein at the cell cortex to facilitate spindle positioning.Cell cycle (Georgetown, Tex.) · 2014Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spindle orientation defines the plane of cell division and, thereby, the spatial position of all daughter cells. Here, we develop a live cell microscopy-based methodology to extract spindle movements in human epithelial cell lines and study how spindles are brought to a pre-defined orientation. We show that spindles undergo two distinct regimes of movements. Spindles are first actively rotated toward the cells' long-axis and then maintained along this pre-defined axis. By quantifying spindle movements in cells depleted of LGN, we show that the first regime of rotational movements requires LGN that recruits cortical dynein. In contrast, the second regime of movements that maintains spindle orientation does not require LGN, but is sensitive to 2ME2 that suppresses microtubule dynamics. Our study sheds first insight into spatially defined spindle movement regimes in human cells, and supports the presence of LGN and dynein independent cortical anchors for astral microtubules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.