ArticleStem cell research2013
Self-renewal and multilineage differentiation of mouse dental epithelial stem cells.
Article in Stem cell research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Resolving tooth development at single-cell resolution: advancing dental regeneration.Frontiers in cell and developmental biology · 2026Review
- Unraveling the hidden complexity: Exploring dental tissues through single-cell transcriptional profiling.Regenerative therapy · 2024Review
- Fgf9 promotes incisor dental epithelial stem cell survival and enamel formation.Stem cell research & therapy · 2024Article
- From Pluripotent Stem Cells to Organoids and Bioprinting: Recent Advances in Dental Epithelium and Ameloblast Models to Study Tooth Biology and Regeneration.Stem cell reviews and reports · 2024Review
- Single-cell census of human tooth development enables generation of human enamel.Developmental cell · 2023Article
- Mesenchymal Stem Cells Derived from Human Periapical Cysts and Their Implications in Regenerative Medicine.Biomedicines · 2023Review
- Activation of Wnt signalling reduces the population of cancer stem cells in ameloblastoma.Cell proliferation · 2021Article
- Intertwined Signaling Pathways Governing Tooth Development: A Give-and-Take Between Canonical Wnt and Shh.Frontiers in cell and developmental biology · 2021Review
- New insight into dental epithelial stem cells: Identification, regulation, and function in tooth homeostasis and repair.World journal of stem cells · 2020Review
- Activation of mesenchymal stem cells promotes new bone formation within dentigerous cyst.Stem cell research & therapy · 2020Article
- The putative role of insulin-like growth factor (IGF)-binding protein 5 independent of IGF in the maintenance of pulpal homeostasis in mice.Regenerative therapy · 2019Article
- Cervical cancer stem cell-associated genes: Prognostic implications in cervical cancer.Oncology letters · 2019Review
- Oct-4 and CD44 in epithelial stem cells like of benign odontogenic lesions.Histochemistry and cell biology · 2018Article
- Runx1-Stat3 signaling regulates the epithelial stem cells in continuously growing incisors.Scientific reports · 2018Article
- Plasticity within the niche ensures the maintenance of aDevelopment (Cambridge, England) · 2018Article
- The Role of Integrin α6 (CD49f) in Stem Cells: More than a Conserved Biomarker.Stem cells and development · 2017Review
- Resolving stem and progenitor cells in the adult mouse incisor through gene co-expression analysis.eLife · 2017Article
- Generation of Spheres from Dental Epithelial Stem Cells.Frontiers in physiology · 2017Article
- Odontogenic epithelial stem cells: hidden sources.Laboratory investigation; a journal of technical methods and pathology · 2015Review
- Activated WNT signaling in postnatal SOX2-positive dental stem cells can drive odontoma formation.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Understanding the cellular and molecular mechanisms underlying the self-renewal and differentiation of dental epithelial stem cells (DESCs) that support the unlimited growth potential of mouse incisors is critical for developing novel tooth regenerative therapies and unraveling the pathogenesis of odontogenic tumors. However, analysis of DESC properties and regulation has been limited by the lack of an in vitro assay system and well-documented DESC markers. Here, we describe an in vitro sphere culture system to isolate the DESCs from postnatal mouse incisor cervical loops (CLs) where the DESCs are thought to reside. The dissociated cells from CLs were able to expand and form spheres for multiple generations in the culture system. Lineage tracing indicated that DESC within the spheres were epithelial in origin as evident by lineage tracing. Upon stimulation, the sphere cells differentiated into cytokeratin 14- and amelogenin-expressing and mineral material-producing cells. Compared to the CL tissue, sphere cells expressed high levels of expression of Sca-1, CD49f (also designated as integrin α6), and CD44. Fluorescence-activated cell sorting (FACS) analyses of mouse incisor CL cells further showed that the CD49f(Bright) population was enriched in sphere-forming cells. In addition, the CD49f(Bright) population includes both slow-cycling and Lgr5(+) DESCs. The in vitro sphere culture system and identification of CD49f(Bright) as a DESC marker provide a novel platform for enriching DESCs, interrogating how maintenance, cell fate determination, and differentiation of DESCs are regulated, and developing tooth regenerative therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.