ArticleAlcoholism, clinical and experimental research2014
Deletion of Prkcz increases intermittent ethanol consumption in mice.
Article in Alcoholism, clinical and experimental research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 26 citations in OpenAlex.
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- Chronic alcohol drinking delays recovery from capsaicin- and nerve injury-induced hypersensitivity in mice.bioRxiv : the preprint server for biology · 2025Article
- Alcohol consumption during early adulthood increases the vulnerability of locus coeruleus neurons and amyloid beta pathology in female APP/PS1 mice.Alcohol (Fayetteville, N.Y.) · 2025Article
- PKMζ alters oxycodone-taking in a dose- and sex-dependent manner.Addiction neuroscience · 2024Article
- Alcohol Consumption During Adolescence Alters the Cognitive Function in Adult Male Mice by Persistently Increasing Levels of DUSP6.Molecular neurobiology · 2024Article
- Sex differences in pre- and post-synaptic glutamate signaling in the nucleus accumbens core.Biology of sex differences · 2023Article
- DNA methylation of the promoter region at the CREB1 binding site is a mechanism for the epigenetic regulation of brain-specific PKMζ.Biochimica et biophysica acta. Gene regulatory mechanisms · 2023Article
- Back-translating GWAS findings to animal models reveals a role for Hgfac and Slc39a8 in alcohol and nicotine consumption.Scientific reports · 2022Article
- Differential regulation of alcohol consumption and reward by the transcriptional cofactor LMO4.Molecular psychiatry · 2021Article
- Nucleus accumbens shell Orexin-1 receptors are not needed for single-bottle limited daily access alcohol intake in C57BL/6 mice.Alcohol (Fayetteville, N.Y.) · 2020Article
- Leveraging Neural Networks in Preclinical Alcohol Research.Brain sciences · 2020Review
- Killing the Buζζ: accumbal PKMζ blunts cocaine seeking and reward.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2019Article
- PKMζ in the nucleus accumbens acts to dampen cocaine seeking.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2018Article
- Novel Small-Molecule Inhibitors of Protein Kinase C Epsilon Reduce Ethanol Consumption in Mice.Biological psychiatry · 2018Article
- Intermittent Access to Ethanol Drinking Facilitates the Transition to Excessive Drinking After Chronic Intermittent Ethanol Vapor Exposure.Alcoholism, clinical and experimental research · 2017Article
- Voluntary co-consumption of alcohol and nicotine: Effects of abstinence, intermittency, and withdrawal in mice.Neuropharmacology · 2016Article
- Molecular mechanisms underlying alcohol-drinking behaviours.Nature reviews. Neuroscience · 2016Review
- Selective chemical genetic inhibition of protein kinase C epsilon reduces ethanol consumption in mice.Neuropharmacology · 2016Article
- Responses to drugs of abuse and non-drug rewards in leptin deficient ob/ob mice.Psychopharmacology · 2016Article
- Genes and Alcohol Consumption: Studies with Mutant Mice.International review of neurobiology · 2016Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPrkcz has been identified as a gene whose expression is positively correlated with ethanol (EtOH) consumption in mice and is also induced by EtOH. Two proteins are produced from Prkcz: protein kinase M zeta (PKMζ), which is expressed in the nervous system and protein kinase C zeta (PKCζ), which is expressed in other tissues. We examined Prkcz(-/-) mice that lack PKCζ and PKMζ to investigate the role of this gene in behavioral responses to EtOH.
methodsMale Prkcz(-/-) and wild-type littermates were tested for EtOH consumption using 4 procedures: 24-hour intermittent access, 4-hour limited intermittent access, 4-day drinking-in-the-dark, and 24-hour continuous access. We also assessed the acute hypnotic effect of EtOH, EtOH reward, and taste preference for sweet-, bitter-, salty-, and umami-flavored solutions. Finally, we determined whether EtOH could increase PKMζ and PKCζ transcripts and protein expression in wild-type mice using quantitative PCR and Western blot analysis.
resultsPrkcz(-/-) mice consumed more EtOH than their wild-type littermates in both intermittent access procedures, but not in the drinking-in-the-dark or 24-hour continuous access procedures. EtOH exposure increased Prkcz transcripts in cultured PC12 cells, and intermittent EtOH consumption increased PKMζ protein in the ventral striatum of wild-type mice.
conclusionsAbsence of PKMζ in the brain is associated with increased EtOH intake during procedures that incorporate intermittent consumption sessions every other day. Our data suggest that EtOH induces PKMζ, which acts in a negative feedback loop to limit binge-like EtOH consumption.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.