ArticleJournal of the American Chemical Society2013
In vitro and cellular self-assembly of a Zn-binding protein cryptand via templated disulfide bonds.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Atomically Accurate Design of Metalloproteins with Predefined Coordination Geometries.Journal of the American Chemical Society · 2023Article
- Design of a Flexible, Zn-Selective Protein Scaffold that Displays Anti-Irving-Williams Behavior.Journal of the American Chemical Society · 2022Article
- Computationally Guided Redesign of a Heme-free Cytochrome with Native-like Structure and Stability.Biochemistry · 2022Article
- A disulphide bond-mediated hetero-dimer of a hemoprotein and a fluorescent protein exhibiting efficient energy transfer.RSC advances · 2022Article
- Redox- and metal-directed structural diversification in designed metalloprotein assemblies.Chemical communications (Cambridge, England) · 2022Article
- Supramolecular assembly of protein building blocks: from folding to function.Nano convergence · 2022Review
- Protein Assembly by Design.Chemical reviews · 2021Review
- Design of metal-mediated protein assemblies via hydroxamic acid functionalities.Nature protocols · 2021Article
- Metal-Templated Design of Chemically Switchable Protein Assemblies with High-Affinity Coordination Sites.Angewandte Chemie (International ed. in English) · 2020Article
- Engineering protein assemblies with allosteric control via monomer fold-switching.Nature communications · 2019Article
- Determining the Structural and Energetic Basis of Allostery in a De Novo Designed Metalloprotein Assembly.Journal of the American Chemical Society · 2018Article
- De Novo Design of an Allosteric Metalloprotein Assembly with Strained Disulfide Bonds.Journal of the American Chemical Society · 2016Article
- Coordination contributions to protein stability in metal-substituted carbonic anhydrase.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2016Article
- Connecting two proteins using a fusion alpha helix stabilized by a chemical cross linker.Nature communications · 2016Article
- Metal-Directed Design of Supramolecular Protein Assemblies.Methods in enzymology · 2016Article
- Domain-swapped cytochromeChemical science · 2015Article
- Self-assembled two-dimensional protein arrays in bionanotechnology: from S-layers to designed lattices.Current opinion in biotechnology · 2014Review
- Protein design: toward functional metalloenzymes.Chemical reviews · 2014Review
- Exceptionally stable, redox-active supramolecular protein assemblies with emergent properties.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Designing hydrolytic zinc metalloenzymes.Biochemistry · 2014Review
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Authors and funding
4 authors.
Funding
Abstract
Simultaneously strong and reversible through redox chemistry, disulfide bonds play a unique and often irreplaceable role in the formation of biological and synthetic assemblies. In an approach inspired by supramolecular chemistry, we report here that engineered noncovalent interactions on the surface of a monomeric protein can template its assembly into a unique cryptand-like protein complex ((C81/C96)RIDC14) by guiding the selective formation of multiple disulfide bonds across different interfaces. Owing to its highly interconnected framework, (C81/C96)RIDC14 is well preorganized for metal coordination in its interior, can support a large internal cavity surrounding the metal sites, and can withstand significant alterations in inner-sphere metal coordination. (C81/C96)RIDC14 self-assembles with high fidelity and yield in the periplasmic space of E. coli cells, where it can successfully compete for Zn(II) binding.
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Registered trials
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