ArticleJournal of clinical and diagnostic research : JCDR2013
PCR as a diagnostic tool for extra-pulmonary tuberculosis.
Article in Journal of clinical and diagnostic research : JCDR, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 29 citations in OpenAlex.
- Incidental lymphadenopathy in renal transplantation.Annals of the Royal College of Surgeons of England · 2024Article
- Evolution of Laboratory Diagnosis of Tuberculosis.Clinics and practice · 2024Review
- Skin test of tuberculin purified protein derivatives with a dissolving microneedle-array patch.Drug delivery and translational research · 2019Article
- Clinical Features and Drug-Resistance Profile of Urinary Tuberculosis in South-Western China: A Cross-sectional Study.Medicine · 2016Article
- Tuberculous pyomyositis: a re-emerging entity of many faces.Clinical rheumatology · 2016Review
- The relevance of biopsy in tuberculosis patients without human immunodeficiency virus infection.The American journal of tropical medicine and hygiene · 2015Article
- Posterior mediastinal tuberculous lymphadenitis with dysphagia as the main symptom: a case report and literature review.Journal of thoracic disease · 2013Article
- Should men with idiopathic obstructive azoospermia be screened for genitourinary tuberculosis?Journal of human reproductive sciencesArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionExtra-Pulmonary Tuberculosis (EPTB) accounts for approximately 40% of the tuberculosis cases. Though it is not communicable, it is a significant cause of morbidity. This study was conducted to know the efficacy of the Polymerase Chain Reaction (PCR) as an additional tool, along with the conventional methods, in the diagnosis of EPTB. MATERIAL AND
methodsClinical samples were collected from suspected cases of EPTB. The Ziehl-Neelsen staining (ZNS), culture on the Lowenstein-Jensen medium (LJM) and PCR testing with the use of a commercial kit were performed on the homogenized samples.
resultsA total of 182 samples which were received for the molecular diagnosis of EPTB were also tested by ZNS and culture on LJM for the presence of Mycobacterium tuberculosis. Of these, 22 were positive by at least one of the tests which were used. PCR detected the maximum number of cases of EPTB, followed by culture. The results of PCR and the conventional tests were analyzed by using McNemar's test for the correlated proportions-the exact method of 'IBM SPSS Statistics 20'. The analysis showed a statistical significance.
conclusionsWhenever they are feasible, using all the available tests in combination increases the laboratory detection rates of M. tuberculosis from clinical samples. PCR must be included in the diagnostic panel of EPTB.
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