ArticlePlant cell reports2013
Arabidopsis CDPK6 phosphorylates ADF1 at N-terminal serine 6 predominantly.
Article in Plant cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- Genome-wide characterization and expression profiling of FIMBRIN gene family members in response to abiotic stress in Medicago sativa.BMC plant biology · 2025Article
- Interdependence of plasma membrane nanoscale dynamics of a kinase and its cognate substrate underlieseLife · 2025Article
- MYB52 negatively regulates ADF9-meditated actin filament bundling in Arabidopsis pavement cell morphogenesis.Journal of integrative plant biology · 2024Article
- Activation of actin-depolymerizing factor by CDPK16-mediated phosphorylation promotes actin turnover in Arabidopsis pollen tubes.PLoS biology · 2023Article
- Actin Depolymerization Factor ADF1 Regulated by MYB30 Plays an Important Role in Plant Thermal Adaptation.International journal of molecular sciences · 2023Article
- Genome-wide identification of actin-depolymerizing factor gene family and their expression patterns under various abiotic stresses in soybean (Frontiers in plant science · 2023Article
- Controlling the Gate: The Functions of the Cytoskeleton in Stomatal Movement.Frontiers in plant science · 2022Review
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- A Decade of Pollen Phosphoproteomics.International journal of molecular sciences · 2021Review
- Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra.Horticulture research · 2021Article
- Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity.Nature communications · 2020Article
- Actin Cytoskeleton as Actor in Upstream and Downstream of Calcium Signaling in Plant Cells.International journal of molecular sciences · 2019Review
- Plant actin depolymerizing factor: actin microfilament disassembly and more.Journal of plant research · 2017Review
- Plant Actin-Depolymerizing Factors Possess Opposing Biochemical Properties Arising from Key Amino Acid Changes throughout Evolution.The Plant cell · 2017Article
- CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing Factor.The Plant cell · 2016Article
- Computational Study of the Binding Mechanism of Actin-Depolymerizing Factor 1 with Actin in Arabidopsis thaliana.PloS one · 2016Article
- Actin-Depolymerizing Factor Subgroup I Members Negatively Regulate Pollen Tube Growth in Fortunella hindsii.Physiologia plantarumArticle
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2 authors.
Funding
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Abstract
key messageWe found that Arabidopsis AtADF1 was phosphorylated by AtCDPK6 at serine 6 predominantly and the phosphoregulation plays a key role in the regulation of ADF1-mediated depolymerization of actin filaments. ABSTRACT: Since actin-depolymerizing factor (ADF) is highly conserved among eukaryotes, it is one of the key modulators for actin organization. In plants, ADF is directly involved in the depolymerization of actin filaments, and therefore important for F-actin-dependent cellular activities. The activity of ADF is tightly controlled through a number of molecular mechanisms, including phosphorylation-mediated inactivation of ADF. To investigate Arabidopsis ADF1 phosphoregulation, we generated AtADF1 phosphorylation site-specific mutants. Using transient expression and stable transgenic approaches, we analyzed the ADF1 phosphorylation mutants in the regulation of actin filament organizations in plant cells. By in vitro phosphorylation assay, we showed that AtADF1 is phosphorylated by AtCDPK6 at serine 6 predominantly. Chemically induced expression of AtCDPK6 can negatively regulate the wild-type AtADF1 in depolymerizing actin filaments, but not those of the mutants AtADF1(S6A) and AtADF1(S6D). These results demonstrate a regulatory function of Arabidopsis CDPK6 in the N-terminal phosphorylation of AtADF1.
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