ArticleJournal of clinical microbiology2013
Identification and subtyping of clinically relevant human and ruminant mycoplasmas by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Advancing standardization of diagnostics and antimicrobial susceptibility testing for pathogenic mycoplasmas of livestock origin: insights from the MyMIC network.BMC veterinary research · 2025Article
- Review
- A Systematic Review of the Recent Techniques Commonly Used in the Diagnosis ofPathogens (Basel, Switzerland) · 2023Review
- Case Report: Double trouble: a rare case of successfully treatedFrontiers in cellular and infection microbiology · 2023Article
- Isolation ofIDCases · 2023Article
- Predominant Single Stable VpmaV Expression in Strain GM139 and Major Differences withAnimals : an open access journal from MDPI · 2022Article
- Review
- Molecular Tools for TypingFrontiers in microbiology · 2022Review
- A Case Report ofFrontiers in medicine · 2022Article
- Postoperative mediastinitis after cardiac surgery caused by Mycoplasma hominis: a case report.Surgical case reports · 2021Article
- Evaluation of Nanopore Sequencing as a Diagnostic Tool for the Rapid Identification of Mycoplasma bovis from Individual and Pooled Respiratory Tract Samples.Journal of clinical microbiology · 2021Article
- MALDI-TOF mass spectrometry and high-resolution melting PCR for the identification of Mycoplasma bovis isolates.BMC veterinary research · 2021Article
- The Brief Case: Mycoplasma hominis Extragenital Abscess.Journal of clinical microbiology · 2021Review
- Addressing the Antimicrobial Resistance of Ruminant Mycoplasmas Using a Clinical Surveillance Network.Frontiers in veterinary science · 2021Article
- Review
- Investigation on eggshell apex abnormality (EAA) syndrome in France: isolation of Mycoplasma synoviae is frequently associated with Mycoplasma pullorum.BMC veterinary research · 2020Article
- Rapid Identification of Mycoplasma bovis Strains from Bovine Bronchoalveolar Lavage Fluid with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry after Enrichment Procedure.Journal of clinical microbiology · 2020Article
- Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Is a Superior Diagnostic Tool for the Identification and Differentiation of Mycoplasmas Isolated from Animals.Journal of clinical microbiology · 2019Article
- Identification and differentiation of avianJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Iatrogenic Ventriculitis Due to Mycoplasma Hominis: A Case Report and Review of the Literature.The American journal of case reports · 2019Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) recently emerged as a technology for the identification of bacteria. In this study, we aimed to evaluate its applicability to human and ruminant mycoplasmal identification, which can be demanding and time-consuming when using phenotypic or molecular methods. In addition, MALDI-TOF MS was tested as a subtyping tool for certain species. A total of 29 main spectra (MSP) from 10 human and 13 ruminant mycoplasma (sub)species were included in a mycoplasma MSP database to complete the Bruker MALDI Biotyper database. After broth culture and protein extraction, MALDI-TOF MS was applied for the identification of 119 human and 143 ruminant clinical isolates that were previously identified by antigenic or molecular methods and for subcultures of 73 ruminant clinical specimens that potentially contained several mycoplasma species. MALDI-TOF MS resulted in accurate (sub)species-level identification with a score of ≥1.700 for 96% (251/262) of the isolates. The phylogenetically closest (sub)species were unequivocally distinguished. Although mixtures of the strains were reliably detected up to a certain cellular ratio, only the predominant species was identified from the cultures of polymicrobial clinical specimens. For typing purposes, MALDI-TOF MS proved to cluster Mycoplasma bovis and Mycoplasma agalactiae isolates by their year of isolation and genome profiles, respectively, and Mycoplasma pneumoniae isolates by their adhesin P1 type. In conclusion, MALDI-TOF MS is a rapid, reliable, and cost-effective method for the routine identification of high-density growing mycoplasmal species and shows promising prospects for its capacity for strain typing.
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