ArticleThe Plant cell2013
Characterization of the early events leading to totipotency in an Arabidopsis protoplast liquid culture by temporal transcript profiling.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 130 citations in OpenAlex.
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Cell-specific dedifferentiation inbioRxiv : the preprint server for biology · 2026Article
- Dedifferentiation of Plant Cells: A Term Covering Multiple Pathways?Plants (Basel, Switzerland) · 2026Article
- Comprehensive transcriptomic and functional characterization of protoplast regeneration in Angelica gigas Nakai.BMC plant biology · 2026Article
- Conserved spatial patterning of gene expression in independent lineages of CThe New phytologist · 2026Article
- Cytokinin-supplemented medium drivesFrontiers in plant science · 2026Article
- Droplet-based microfluidics platform for investigation of protoplast development of three exemplary plant species.Scientific reports · 2025Article
- STEMIN transcription factor drives selective chromatin remodeling for gene activation within a relaxed chromatin during reprogramming in the moss Physcomitrium patens.The Plant journal : for cell and molecular biology · 2025Article
- Dual and spatially resolved drought responses in the Arabidopsis leaf mesophyll revealed by single-cell transcriptomics.The New phytologist · 2025Article
- The cell colony development is connected with the accumulation of embryogenesis-related proteins and dynamic distribution of cell wall components in in vitro cultures of Fagopyrum tataricum and Fagopyrum esculentum.BMC plant biology · 2025Article
- Developmental regulatory genes in recalcitrant forest trees: advances in somatic regeneration and genetic transformation.Frontiers in plant science · 2025Review
- Multiscale chromatin dynamics and high entropy in plant iPSC ancestors.Journal of cell science · 2024Article
- Plant regeneration in the new era: from molecular mechanisms to biotechnology applications.Science China. Life sciences · 2024Review
- Response surface methodology mediated optimization of phytosulfokine and plant growth regulators for enhanced protoplast division, callus induction, and somatic embryogenesis in Angelica Gigas Nakai.BMC plant biology · 2024Article
- A cell-based fluorescent system and statistical framework to detect meiosis-like induction in plants.Frontiers in plant science · 2024Article
- A Common Molecular Signature Indicates the Pre-Meristematic State of Plant Calli.International journal of molecular sciences · 2023Review
- Molecular Mechanisms of Plant Regeneration from Differentiated Cells: Approaches from Historical Tissue Culture Systems.Plant & cell physiology · 2023Review
- Overcoming roadblocks forFrontiers in plant science · 2023Review
- Transcriptome Dynamics of Rooting Zone and Leaves during In Vitro Adventitious Root Formation inPlants (Basel, Switzerland) · 2022Article
- Transcriptional activation of auxin biosynthesis drives developmental reprogramming of differentiated cells.The Plant cell · 2022Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular mechanisms underlying plant cell totipotency are largely unknown. Here, we present a protocol for the efficient regeneration of plants from Arabidopsis thaliana protoplasts. The specific liquid medium used in our study leads to a high rate of reentry into the cell cycle of most cell types, providing a powerful system to study dedifferentiation/regeneration processes in independent somatic cells. To identify the early events in the establishment of totipotency, we monitored the genome-wide transcript profiles of plantlets and protoplast-derived cells (PdCs) during the first week of culture. Plant cells rapidly dedifferentiated. Then, we observed the reinitiation and reorientation of protein synthesis, accompanied by the reinitiation of cell division and de novo cell wall synthesis. Marked changes in the expression of chromatin-associated genes, especially of those in the histone variant family, were observed during protoplast culture. Surprisingly, the epigenetic status of PdCs and well-established cell cultures differed, with PdCs exhibiting rare reactivated transposons and epigenetic changes. The differentially expressed genes identified in this study are interesting candidates for investigating the molecular mechanisms underlying plant cell plasticity and totipotency. One of these genes, the plant-specific transcription factor ABERRANT LATERAL ROOT FORMATION4, is required for the initiation of protoplast division.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.