ArticleThe Plant cell2013
Multisite light-induced phosphorylation of the transcription factor PIF3 is necessary for both its rapid degradation and concomitant negative feedback modulation of photoreceptor phyB levels in Arabidopsis.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed, 155 citations in OpenAlex.
- Transcriptional dynamics and chromatin accessibility in the regulation of shade-responsive genes in Arabidopsis.Genome biology · 2025Article
- Involvement of Phytochrome-Interacting Factors in High-Irradiance Adaptation.International journal of molecular sciences · 2025Article
- Gibberellin transport affects lateral root growth through HY5 in response to far-red light.The Plant cell · 2025Article
- Both phytochrome A and phyB interact with PHYTOCHROME-INTERACTING FACTORs through an evolutionary conserved phyNature communications · 2025Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Light signal regulates endoreduplication and tomato fruit expansion through the SlPIF1a-SlTLFP8-SlCDKB2 module.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- NPR1 promotes blue light-induced plant photomorphogenesis by ubiquitinating and degrading PIF4.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- PHOSPHATASE 2A dephosphorylates PHYTOCHROME-INTERACTING FACTOR3 to modulate photomorphogenesis in Arabidopsis.The Plant cell · 2024Article
- A circadian clock output functions independently of phyB to sustain daytime PIF3 degradation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- What is going on inside of phytochrome B photobodies?The Plant cell · 2024Review
- The phosphorylation of carboxyl-terminal eIF2α by SPA kinases contributes to enhanced translation efficiency during photomorphogenesis.Nature communications · 2024Article
- Article
- Phytochromes enhance SOS2-mediated PIF1 and PIF3 phosphorylation and degradation to promote Arabidopsis salt tolerance.The Plant cell · 2023Article
- Linking discoveries, mechanisms, and technologies to develop a clearer perspective on plant long noncoding RNAs.The Plant cell · 2023Review
- Type one protein phosphatase regulates fixed-carbon starvation-induced autophagy in Arabidopsis.The Plant cell · 2022Article
- Shade triggers posttranscriptional PHYTOCHROME-INTERACTING FACTOR-dependent increases in H3K4 trimethylation.Plant physiology · 2022Article
- Phosphorylation of MdERF17 by MdMPK4 promotes apple fruit peel degreening during light/dark transitions.The Plant cell · 2022Article
- WRKY2 and WRKY10 regulate the circadian expression ofPlant communications · 2022Article
- Chromatin Changes in Phytochrome Interacting Factor-Regulated Genes Parallel Their Rapid Transcriptional Response to Light.Frontiers in plant science · 2022Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Plants constantly monitor informational light signals using sensory photoreceptors, which include the phytochrome (phy) family (phyA to phyE), and adjust their growth and development accordingly. Following light-induced nuclear translocation, photoactivated phy molecules bind to and induce rapid phosphorylation and degradation of phy-interacting basic Helix Loop Helix (bHLH) transcription factors (PIFs), such as PIF3, thereby regulating the expression of target genes. However, the mechanisms underlying the signal-relay process are still not fully understood. Here, using mass spectrometry, we identify multiple, in vivo, light-induced Ser/Thr phosphorylation sites in PIF3. Using transgenic expression of site-directed mutants of PIF3, we provide evidence that a set of these phosphorylation events acts collectively to trigger rapid degradation of the PIF3 protein in response to initial exposure of dark-grown seedlings to light. In addition, we show that phyB-induced PIF3 phosphorylation is also required for the known negative feedback modulation of phyB levels in prolonged light, potentially through codegradation of phyB and PIF3. This mutually regulatory intermolecular transaction thus provides a mechanism with the dual capacity to promote early, graded, or threshold regulation of the primary, PIF3-controlled transcriptional network in response to initial light exposure, and later, to attenuate global sensitivity to the light signal through reductions in photoreceptor levels upon prolonged exposure.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.