ArticleDevelopment (Cambridge, England)2013
Phosphorylation of RNA polymerase II is independent of P-TEFb in the C. elegans germline.
Article in Development (Cambridge, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Dual Modes of Gene Regulation by CDK12.bioRxiv : the preprint server for biology · 2025Article
- Interdependence of Pasha and Drosha for localization and function of the Microprocessor in C. elegans.Nature communications · 2025Article
- The TOP-2/condensin II axis silences transcription during germline specification in C. elegans.G3 (Bethesda, Md.) · 2024Article
- Regulation of Microprocessor assembly and localization via Pasha's WW domain inbioRxiv : the preprint server for biology · 2024Article
- Characterization of factors that underlie transcriptional silencing in C. elegans oocytes.PLoS genetics · 2023Article
- Transcriptional repression during spermatogenesis inmicroPublication biology · 2023Article
- Article
- A DNA replication-independent function of pre-replication complex genes during cell invasion in C. elegans.PLoS biology · 2022Article
- DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.PLoS genetics · 2021Article
- RNA polymerase II CTD S2P is dispensable for embryogenesis but mediates exit from developmental diapause inScience advances · 2020Article
- CDK12 globally stimulates RNA polymerase II transcription elongation and carboxyl-terminal domain phosphorylation.Nucleic acids research · 2020Article
- DNA Damage Induces Dynamic Associations of BRD4/P-TEFb With Chromatin and Modulates Gene Transcription in a BRD4-Dependent and -Independent Manner.Frontiers in molecular biosciences · 2020Article
- Article
- Multiple roles of CTDK-I throughout the cell.Cellular and molecular life sciences : CMLS · 2019Review
- Human CDK12 and CDK13, multi-tasking CTD kinases for the new millenium.Transcription · 2019Review
- AMPK blocks starvation-inducible transgenerational defects inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- The pol II CTD: new twists in the tail.Nature structural & molecular biology · 2016Review
- A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans.PLoS genetics · 2016Article
- Article
- Functional interaction of Rpb1 and Spt5 C-terminal domains in co-transcriptional histone modification.Nucleic acids research · 2015Article
Corrections and comments
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4 authors.
Funding
Abstract
RNA polymerase II (Pol II) elongation in metazoans is thought to require phosphorylation of serine 2 (Ser2-P) of the Pol II C-terminal domain (CTD) by the P-TEFb complex, CDK-9/cyclin T. Another Ser2 kinase complex, CDK-12/cyclin K, which requires upstream CDK-9 activity has been identified in Drosophila and human cells. We show that regulation of Ser2-P in C. elegans soma is similar to other metazoan systems, but Ser2-P in the germline is independent of CDK-9, and largely requires only CDK-12. The observed differences are not due to differential tissue expression as both kinases and their cyclin partners are ubiquitously expressed. Surprisingly, loss of CDK-9 from germ cells has little effect on Ser2-P, yet CDK-9 is essential for germline development. By contrast, loss of CDK-12 and Ser2-P specifically from germ cells has little impact on germline development or function, although significant loss of co-transcriptional H3K36 trimethylation is observed. These results show a reduced requirement for Pol II Ser2-P in germline development and suggest that generating Ser2-P is not the essential role of CDK-9 in these cells. Transcriptional elongation in the C. elegans germline thus appears to be uniquely regulated, which may be a novel facet of germline identity.
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