ArticleParticle and fibre toxicology2013
Multi-walled carbon nanotubes induce human microvascular endothelial cellular effects in an alveolar-capillary co-culture with small airway epithelial cells.
Article in Particle and fibre toxicology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 89 citations in OpenAlex.
- MMP-3-mediated cleavage of OPN is involved in copper oxide nanoparticle-induced activation of fibroblasts.Particle and fibre toxicology · 2023Article
- Carbon Nanotubes as Carriers in Drug Delivery for Non-Small Cell Lung Cancer, Mechanistic Analysis of Their Carcinogenic Potential, Safety Profiling and Identification of Biomarkers.International journal of nanomedicine · 2022Review
- Single-cell patterning technology for biological applications.Biomicrofluidics · 2019Review
- Acquisition of Cancer Stem Cell-like Properties in Human Small Airway Epithelial Cells after a Long-term Exposure to Carbon Nanomaterials.Environmental science. Nano · 2019Article
- Biofabrication of phenotypic pulmonary fibrosis assays.Biofabrication · 2019Review
- Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure.Toxicology reports · 2019Article
- Comparative study of in vitro effects of different nanoparticles at non-cytotoxic concentration on the adherens junction of human vascular endothelial cells.International journal of nanomedicine · 2019Article
- Understanding and improving assays for cytotoxicity of nanoparticles: what really matters?RSC advances · 2018Article
- Cytotoxicological pathways induced after nanoparticle exposure: studies of oxidative stress at the 'nano-bio' interface.Toxicology research · 2017Review
- Human barrier models for the in vitro assessment of drug delivery.Drug delivery and translational research · 2017Article
- ICAM-1-Targeted Nanocarriers Attenuate Endothelial Release of Soluble ICAM-1, an Inflammatory Regulator.Bioengineering & translational medicine · 2017Article
- Carbon Nanotubes Induced Fibrogenesis on Nanostructured Substrates.Environmental science. Nano · 2017Article
- Cellular crosstalk between airway epithelial and endothelial cells regulates barrier functions during exposure to double-stranded RNA.Immunity, inflammation and disease · 2017Article
- Effects of lipopolysaccharide, multiwalled carbon nantoubes, and the combination on lung alveolar epithelial cells.Environmental toxicology · 2017Article
- Thrombospondin-1 mediates multi-walled carbon nanotube induced impairment of arteriolar dilation.Nanotoxicology · 2017Article
- Critical review of the current and future challenges associated with advanced in vitro systems towards the study of nanoparticle (secondary) genotoxicity.Mutagenesis · 2017Review
- Biological mechanisms of gold nanoparticle radiosensitization.Cancer nanotechnology · 2017Review
- Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo.International journal of nanomedicine · 2017Article
- Induction of cancer-associated fibroblast-like cells by carbon nanotubes dictates its tumorigenicity.Scientific reports · 2016Article
- Using cultured endothelial cells to study endothelial barrier dysfunction: Challenges and opportunities.American journal of physiology. Lung cellular and molecular physiology · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundNanotechnology, particularly the use of multi-walled carbon nanotubes (MWCNT), is a rapidly growing discipline with implications for advancement in a variety of fields. A major route of exposure to MWCNT during both occupational and environmental contact is inhalation. While many studies showed adverse effects to the vascular endothelium upon MWCNT exposure, in vitro results often do not correlate with in vivo effects. This study aimed to determine if an alveolar-capillary co-culture model could determine changes in the vascular endothelium after epithelial exposure to MWCNT.
methodsA co-culture system in which both human small airway epithelial cells and human microvascular endothelial cells were separated by a Transwell membrane so as to resemble an alveolar-capillary interaction was used. Following exposure of the epithelial layer to MWCNT, the effects to the endothelial barrier were determined.
resultsExposure of the epithelial layer to MWCNT induced multiple changes in the endothelial cell barrier, including an increase in reactive oxygen species, actin rearrangement, loss of VE-cadherin at the cell surface, and an increase in endothelial angiogenic ability. Overall increases in secreted VEGFA, sICAM-1, and sVCAM-1 protein levels, as well as increases in intracellular phospho-NF-κB, phospho-Stat3, and phospho-p38 MAPK, were also noted in HMVEC after epithelial exposure.
conclusionThe co-culture system identified that alveolar-capillary exposure to MWCNT induced multiple changes to the underlying endothelium, potentially through cell signaling mediators derived from MWCNT-exposed epithelial cells. Therefore, the co-culture system appears to be a relevant in vitro method to study the pulmonary toxicity of MWCNT.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.