ArticleJournal of cell science2013
Phosphorylation of chemoattractant receptors regulates chemotaxis, actin reorganization and signal relay.
Article in Journal of cell science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Dictyostelium discoideum chemotaxis is altered by hypoxia to orient streaming toward higher oxygen levels.BMC molecular and cell biology · 2025Article
- cAMP-PKA/EPAC signaling and cancer: the interplay in tumor microenvironment.Journal of hematology & oncology · 2024Review
- Toxoplasma ERK7 protects the apical complex from premature degradation.The Journal of cell biology · 2023Article
- An integrated, cross-regulation pathway model involving activating/adaptive and feed-forward/feed-back loops for directed oscillatory cAMP signal-relay/response during the development ofFrontiers in cell and developmental biology · 2023Article
- Altered CXCR4 dynamics at the cell membrane impairs directed cell migration in WHIM syndrome patients.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Different Heterotrimeric G Protein Dynamics for Wide-Range Chemotaxis in Eukaryotic Cells.Frontiers in cell and developmental biology · 2021Review
- Loss of a conserved MAPK causes catastrophic failure in assembly of a specialized cilium-like structure inMolecular biology of the cell · 2020Article
- DPF is a cell-density sensing factor, with cell-autonomous and non-autonomous functions during Dictyostelium growth and development.BMC biology · 2019Article
- The Atypical MAP Kinase ErkB Transmits Distinct Chemotactic Signals through a Core Signaling Module.Developmental cell · 2019Article
- Decoding the chemotactic signal.Journal of leukocyte biology · 2018Review
- Cell motility in cancer invasion and metastasis: insights from simple model organisms.Nature reviews. Cancer · 2018Review
- G-Protein Dependent Signal Transduction and Ubiquitination in Dictyostelium.International journal of molecular sciences · 2017Review
- A module for Rac temporal signal integration revealed with optogenetics.The Journal of cell biology · 2017Article
- Chemotactic network responses to live bacteria show independence of phagocytosis from chemoreceptor sensing.eLife · 2017Article
- Fold-change detection and scale invariance of cell-cell signaling in social amoeba.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Self-organization of chemoattractant waves in Dictyostelium depends on F-actin and cell-substrate adhesion.Journal of the Royal Society, Interface · 2016Article
- Gβ promotes pheromone receptor polarization and yeast chemotropism by inhibiting receptor phosphorylation.Science signaling · 2016Article
- Function and Regulation of Heterotrimeric G Proteins during Chemotaxis.International journal of molecular sciences · 2016Review
- The Dictyostelium prestalk inducer differentiation-inducing factor-1 (DIF-1) triggers unexpectedly complex global phosphorylation changes.Molecular biology of the cell · 2015Article
- Chemotactic signaling in mesenchymal cells compared to amoeboid cells.Genes & diseases · 2014Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Migratory cells, including mammalian leukocytes and Dictyostelium, use G-protein-coupled receptor (GPCR) signaling to regulate MAPK/ERK, PI3K, TORC2/AKT, adenylyl cyclase and actin polymerization, which collectively direct chemotaxis. Upon ligand binding, mammalian GPCRs are phosphorylated at cytoplasmic residues, uncoupling G-protein pathways, but activating other pathways. However, connections between GPCR phosphorylation and chemotaxis are unclear. In developing Dictyostelium, secreted cAMP serves as a chemoattractant, with extracellular cAMP propagated as oscillating waves to ensure directional migratory signals. cAMP oscillations derive from transient excitatory responses of adenylyl cyclase, which then rapidly adapts. We have studied chemotactic signaling in Dictyostelium that express non-phosphorylatable cAMP receptors and show through chemotaxis modeling, single-cell FRET imaging, pure and chimeric population wavelet quantification, biochemical analyses and TIRF microscopy, that receptor phosphorylation is required to regulate adenylyl cyclase adaptation, long-range oscillatory cAMP wave production and cytoskeletal actin response. Phosphorylation defects thus promote hyperactive actin polymerization at the cell periphery, misdirected pseudopodia and the loss of directional chemotaxis. Our data indicate that chemoattractant receptor phosphorylation is required to co-regulate essential pathways for migratory cell polarization and chemotaxis. Our results significantly extend the understanding of the function of GPCR phosphorylation, providing strong evidence that this evolutionarily conserved mechanism is required in a signal attenuation pathway that is necessary to maintain persistent directional movement of Dictyostelium, neutrophils and other migratory cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.