ArticleJournal of the American Chemical Society2013
Visualization of peroxynitrite-induced changes of labile Zn2+ in the endoplasmic reticulum with benzoresorufin-based fluorescent probes.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 105 citations in OpenAlex.
- Tuning SBDs as Endoplasmic Reticulum Self-Targeting Fluorophores and Its Application for ZnChemical & biomedical imaging · 2025Article
- Fluorescent Turn-On Sensing of Zinc(II) and Alkaline Phosphatase Activity Using a Pyridoxal-5'-Phosphate Derived Schiff Base.Journal of fluorescence · 2023Article
- Crystal structure of (7-{[bis-(pyridin-2-ylmeth-yl)amino-κActa crystallographica. Section E, Crystallographic communications · 2022Article
- Tools and techniques for illuminating the cell biology of zinc.Biochimica et biophysica acta. Molecular cell research · 2021Review
- Subcellular localised small molecule fluorescent probes to image mobile ZnChemical science · 2020Review
- A rationally designed peptoid for the selective chelation of ZnChemical science · 2020Article
- Fluorescent detection of peroxynitrite during antibody-dependent cellular phagocytosis.Methods in enzymology · 2020Article
- Endoplasmic reticulum targeting fluorescent probes to image mobile ZnChemical science · 2019Article
- Targeting Fluorescent Sensors to Endoplasmic Reticulum Membranes Enables Detection of Peroxynitrite During Cellular Phagocytosis.ACS chemical biology · 2018Article
- Green and Red Fluorescent Dyes for Translational Applications in Imaging and Sensing Analytes: A Dual-Color Flag.ChemistryOpen · 2018Review
- Neuronal Nitric Oxide Synthase Contributes to PTZ Kindling Epilepsy-Induced Hippocampal Endoplasmic Reticulum Stress and Oxidative Damage.Frontiers in cellular neuroscience · 2017Article
- A Simple and Selective Fluorescent Sensor for Zn(2+) and H(+) Ions in Aqueous Solution with OR Logic Gate Function.Journal of fluorescence · 2016Article
- A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection.Biosensors & bioelectronics · 2016Article
- Reaction-Based Probes for Imaging Mobile Zinc in Live Cells and Tissues.ACS sensors · 2016Article
- Obesity-Induced Inflammation Is Associated with Alterations in Subcellular Zinc Pools and Premature Mammary Gland Involution in Lactating Mice.The Journal of nutrition · 2015Article
- Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.Biosensors · 2015Review
- Bi-8-carboxamidoquinoline derivatives for the fluorescent recognition of Zn2+.Journal of fluorescence · 2015Article
- Biologically targeted probes for ZnChemical science · 2014Article
- Addition of a second binding site increases the dynamic range but alters the cellular localization of a red fluorescent probe for mobile zinc.Inorganic chemistry · 2014Article
- The smart 2-(2-fluorobenzoyl)-N-(2-methoxyphenyl)hydrazinecarbothioamide functionalized as Ni(II) sensor in micromolar concentration level and its application in live cell imaging.Journal of fluorescence · 2014Article
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Authors and funding
3 authors at 2 institutions in 1 country.
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Abstract
Zn(2+) plays essential roles in biology, and the homeostasis of Zn(2+) is tightly regulated in all cells. Subcellular distribution and trafficking of labile Zn(2+), and its inter-relation with reactive nitrogen species, are poorly understood due to the scarcity of appropriate imaging tools. We report a new family of red-emitting fluorescent sensors for labile Zn(2+), ZBR1-3, based on a benzoresorufin platform functionalized with dipicolylamine or picolylamine-derived metal binding groups. In combination, the pendant amines and fluorophore afford an [N3O] binding motif that resembles that of previously reported fluorescein-based sensors of the Zinpyr family, reproducing well their binding capabilities and yielding comparable Kd values in the sub-nanomolar and picomolar ranges. The ZBR sensors display up to 8.4-fold emission fluorescence enhancement upon Zn(2+) binding in the cuvette, with similar responses obtained in live cells using standard wide-field fluorescence microscopy imaging. The new sensors localize spontaneously in the endoplasmic reticulum (ER) of various tested cell lines, allowing for organelle-specific monitoring of zinc levels in live cells. Study of ER zinc levels in neural stem cells treated with a peroxynitrite generator, Sin-1, revealed an immediate decrease in labile Zn(2+) thus providing evidence for a direct connection between ER stress and ER Zn(2+) homeostasis.
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