ArticleBMC genomics2013
Systems perspectives on erythromycin biosynthesis by comparative genomic and transcriptomic analyses of S. erythraea E3 and NRRL23338 strains.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Branched-Chain Amino Acids Mediate the Regulation of SACE_Lrp on the Aminotransferase IlvE Involved in Erythromycin Biosynthesis in Saccharopolyspora erythraea.Microbial biotechnology · 2026Article
- dTSR enables efficient improvement of heterologous production of spinosad inSynthetic and systems biotechnology · 2025Article
- Improving butenyl-spinosyn production inFrontiers in microbiology · 2025Article
- Transcriptomics-guided optimization of vitamins to enhance erythromycin yield in saccharopolyspora erythraea.Bioresources and bioprocessing · 2024Article
- CRISPR/Cas9-Mediated Multi-Locus Promoter Engineering inMicroorganisms · 2023Article
- Improvement of macrolactins production by the genetic adaptation of Bacillus siamensis A72 to saline stress via adaptive laboratory evolution.Microbial cell factories · 2022Article
- PhoP- and GlnR-mediated regulation of metK transcription and its impact upon S-adenosyl-methionine biosynthesis in Saccharopolyspora erythraea.Microbial cell factories · 2022Article
- Reconstruction of the Genome-Scale Metabolic Model ofMetabolites · 2022Article
- Carbon catabolite regulation of secondary metabolite formation, an old but not well-established regulatory system.Microbial biotechnology · 2022Review
- Droplet-Microfluidic-Based Promoter Engineering and Expression Fine-Tuning for Improved Erythromycin Production inFrontiers in bioengineering and biotechnology · 2022Article
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- Reconstruction of Secondary Metabolic Pathway to Synthesize Novel Metabolite inFrontiers in bioengineering and biotechnology · 2021Article
- Impact on Multiple Antibiotic Pathways Reveals MtrA as a Master Regulator of Antibiotic Production inApplied and environmental microbiology · 2020Article
- The Expression of NOX From Synthetic Promoters Reveals an Important Role of the Redox Status in Regulating Secondary Metabolism ofFrontiers in bioengineering and biotechnology · 2020Article
- Engineering the Erythromycin-Producing StrainFrontiers in microbiology · 2020Article
- Phosphate regulator PhoP directly and indirectly controls transcription of the erythromycin biosynthesis genes in Saccharopolyspora erythraea.Microbial cell factories · 2019Article
- PccD Regulates Branched-Chain Amino Acid Degradation and Exerts a Negative Effect on Erythromycin Production in Saccharopolyspora erythraea.Applied and environmental microbiology · 2018Article
- TetR Family Transcriptional Regulator PccD Negatively Controls Propionyl Coenzyme A Assimilation in Saccharopolyspora erythraea.Journal of bacteriology · 2017Article
- Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea.Microbial cell factories · 2016Article
- Random transposon mutagenesis of the Saccharopolyspora erythraea genome reveals additional genes influencing erythromycin biosynthesis.FEMS microbiology letters · 2015Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundS. erythraea is a Gram-positive filamentous bacterium used for the industrial-scale production of erythromycin A which is of high clinical importance. In this work, we sequenced the whole genome of a high-producing strain (E3) obtained by random mutagenesis and screening from the wild-type strain NRRL23338, and examined time-series expression profiles of both E3 and NRRL23338. Based on the genomic data and transcriptpmic data of these two strains, we carried out comparative analysis of high-producing strain and wild-type strain at both the genomic level and the transcriptomic level.
resultsWe observed a large number of genetic variants including 60 insertions, 46 deletions and 584 single nucleotide variations (SNV) in E3 in comparison with NRRL23338, and the analysis of time series transcriptomic data indicated that the genes involved in erythromycin biosynthesis and feeder pathways were significantly up-regulated during the 60 hours time-course. According to our data, BldD, a previously identified ery cluster regulator, did not show any positive correlations with the expression of ery cluster, suggesting the existence of alternative regulation mechanisms of erythromycin synthesis in S. erythraea. Several potential regulators were then proposed by integration analysis of genomic and transcriptomic data.
conclusionThis is a demonstration of the functional comparative genomics between an industrial S. erythraea strain and the wild-type strain. These findings help to understand the global regulation mechanisms of erythromycin biosynthesis in S. erythraea, providing useful clues for genetic and metabolic engineering in the future.
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