ArticleScientific reports2013
Affinity flow fractionation of cells via transient interactions with asymmetric molecular patterns.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- Dynamic Microstructured Thermoresponsive Interfaces for Label-Free Cell Sorting Based on Nonspecific Interactions.ACS applied materials & interfaces · 2025Article
- Harnessing the power of Microscale AcoustoFluidics: A perspective based on BAW cancer diagnostics.Biomicrofluidics · 2024Article
- Passive redirection filters minimize red blood cell contamination during neutrophil chemotaxis assays using whole blood.Lab on a chip · 2023Article
- Enhancing size based size separation through vertical focus microfluidics using secondary flow in a ridged microchannel.Scientific reports · 2017Article
- Continuous Sorting of Cells Based on Differential P Selectin Glycoprotein Ligand Expression Using Molecular Adhesion.Analytical chemistry · 2017Article
- Big insights from small volumes: deciphering complex leukocyte behaviors using microfluidics.Journal of leukocyte biology · 2016Review
- Rapid and label-free microfluidic neutrophil purification and phenotyping in diabetes mellitus.Scientific reports · 2016Article
- Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation.Lab on a chip · 2015Review
- Fundamentals and application of magnetic particles in cell isolation and enrichment: a review.Reports on progress in physics. Physical Society (Great Britain) · 2015Review
- Cell separation using tilted-angle standing surface acoustic waves.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Continuous-flow microfluidic blood cell sorting for unprocessed whole blood using surface-micromachined microfiltration membranes.Lab on a chip · 2014Article
- Microfluidic blood cell sorting: now and beyond.Small (Weinheim an der Bergstrasse, Germany) · 2014Review
- Micro total analysis systems: fundamental advances and biological applications.Analytical chemistry · 2014Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Flow fractionation of cells using physical fields to achieve lateral displacement finds wide applications, but its extension to surface molecule-specific separation requires labeling. Here we demonstrate affinity flow fractionation (AFF) where weak, short-range interactions with asymmetric molecular patterns laterally displace cells in a continuous, label-free process. We show that AFF can directly draw neutrophils out of a continuously flowing stream of blood with an unprecedented 400,000-fold depletion of red blood cells, with the sorted cells being highly viable, unactivated, and functionally intact. The lack of background erythrocytes enabled the use of AFF for direct enumeration of neutrophils by a downstream detector, which could distinguish the activation state of neutrophils in blood. The compatibility of AFF with capillary microfluidics and its ability to directly separate cells with high purity and minimal sample preparation will facilitate the design of simple and portable devices for point-of-care diagnostics and quick, cost-effective laboratory analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.