ArticlePlant physiology2013
PYR/PYL/RCAR abscisic acid receptors regulate K+ and Cl- channels through reactive oxygen species-mediated activation of Ca2+ channels at the plasma membrane of intact Arabidopsis guard cells.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 111 citations in OpenAlex.
- A guard cell carbonic anhydrase binds and regulates SLAC1 separate from its catalytic activity.Nature communications · 2026Article
- Roles of ROS and NO in Plant Responses to Individual and Combined Salt Stress and Waterlogging.Antioxidants (Basel, Switzerland) · 2025Review
- SAL1-PAP retrograde signaling orchestrates photosynthetic and extracellular reactive oxygen species for stress responses.The Plant journal : for cell and molecular biology · 2025Article
- Genome-wide identification, abiotic stress, and expression analysis of PYL family in Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) during grain development.BMC plant biology · 2024Article
- Dynamic interplay of reactive oxygen and nitrogen species (ROS and RNS) in plant resilience: unveiling the signaling pathways and metabolic responses to biotic and abiotic stresses.Plant cell reports · 2024Review
- A charged existence: A century of transmembrane ion transport in plants.Plant physiology · 2024Review
- Exogenous application of pectin triggers stomatal closure and immunity in Arabidopsis.Molecular plant pathology · 2024Article
- Chloride salt enhances plant resistance to biotic stresses.Frontiers in plant science · 2024Article
- OnGuard3e: A predictive, ecophysiology-ready tool for gas exchange and photosynthesis research.Plant, cell & environment · 2023Article
- Stomatal Responses of Two Drought-Tolerant Barley Varieties with Different ROS Regulation Strategies under Drought Conditions.Antioxidants (Basel, Switzerland) · 2023Article
- Article
- Molecular Evolution of Calcium Signaling and Transport in Plant Adaptation to Abiotic Stress.International journal of molecular sciences · 2021Review
- Genome-wide identification of ABA receptor PYL/RCAR gene family and their response to cold stress inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Membrane voltage as a dynamic platform for spatiotemporal signaling, physiological, and developmental regulation.Plant physiology · 2021Article
- SAUR proteins and PP2C.D phosphatases regulate H+-ATPases and K+ channels to control stomatal movements.Plant physiology · 2021Article
- Abscisic Acid Mediates Drought and Salt Stress Responses inInternational journal of molecular sciences · 2020Review
- CIPK23 regulates blue light-dependent stomatal opening in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2020Article
- Identification, Evolutionary and Expression Analysis ofPlants (Basel, Switzerland) · 2020Article
- Genome-wide identification and characterization of ABA receptor PYL gene family in rice.BMC genomics · 2020Article
- Stomatal immunity against fungal invasion comprises not only chitin-induced stomatal closure but also chitosan-induced guard cell death.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The discovery of the START family of abscisic acid (ABA) receptors places these proteins at the front of a protein kinase/phosphatase signal cascade that promotes stomatal closure. The connection of these receptors to Ca(2+) signals evoked by ABA has proven more difficult to resolve, although it has been implicated by studies of the pyrbactin-insensitive pyr1/pyl1/pyl2/pyl4 quadruple mutant. One difficulty is that flux through plasma membrane Ca(2+) channels and Ca(2+) release from endomembrane stores coordinately elevate cytosolic free Ca(2+) concentration ([Ca(2+)]i) in guard cells, and both processes are facilitated by ABA. Here, we describe a method for recording Ca(2+) channels at the plasma membrane of intact guard cells of Arabidopsis (Arabidopsis thaliana). We have used this method to resolve the loss of ABA-evoked Ca(2+) channel activity at the plasma membrane in the pyr1/pyl1/pyl2/pyl4 mutant and show the consequent suppression of [Ca(2+)]i increases in vivo. The basal activity of Ca(2+) channels was not affected in the mutant; raising the concentration of Ca(2+) outside was sufficient to promote Ca(2+) entry, to inactivate current carried by inward-rectifying K(+) channels and to activate current carried by the anion channels, both of which are sensitive to [Ca(2+)]i elevations. However, the ABA-dependent increase in reactive oxygen species (ROS) was impaired. Adding the ROS hydrogen peroxide was sufficient to activate the Ca(2+) channels and trigger stomatal closure in the mutant. These results offer direct evidence of PYR/PYL/RCAR receptor coupling to the activation by ABA of plasma membrane Ca(2+) channels through ROS, thus affecting [Ca(2+)]i and its regulation of stomatal closure.
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