ArticleGut and liver2013
Investigation of interleukin-10 promoter polymorphisms and interleukin-10 levels in children with irritable bowel syndrome.
Article in Gut and liver, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01131442 (Immunological Change and the Single Nucleotide Polymorphism), which is not on this map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Immunological Change and the Single Nucleotide Polymorphism (SNP) in Children With Irritable Bowel Syndrome (IBS)
Who cites it
6 citing papers in PubMed, 13 citations in OpenAlex.
- Inflammatory Markers in Children and Adolescents with Functional Somatic Disorders: A Systematic Review.Children (Basel, Switzerland) · 2024Review
- The effects ofPeerJ · 2022Article
- Lack of association between interleukin-10, transforming growth factor-beta gene polymorphisms and juvenile-onset systemic lupus erythematosus.Clinical rheumatology · 2015Article
- Irritable bowel syndrome in children: pathogenesis, diagnosis and evidence-based treatment.World journal of gastroenterology · 2014Review
- Molecular basis of the irritable bowel syndrome.World journal of gastroenterology · 2014Review
- Current insights into the innate immune system dysfunction in irritable bowel syndrome.Annals of gastroenterologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND/
aimsThe aim of this study was to investigate whether genetic variations at positions -1082, -819, and -592 in the interleukin (IL)-10 promoter affect IL-10 production in children with irritable bowel syndrome (IBS).
methodsNinety-four children with IBS and 102 children as healthy controls (HCs) were enrolled. Genomic DNA was extracted, and IL-10 -1082, -819, and -592 polymorphisms were detected by direct sequencing from all participants. Peripheral blood mononuclear cells (PBMCs) from 46 IBS children and 38 HCs were isolated and cultured with and without 5 ng/mL Escherichia coli lipopolysaccharide (LPS). IL-10 levels in the culture supernatants were measured by enzyme-linked immunosorbent assay.
resultsThere were no significant differences in the distribution of IL-10 -1082, -819, and -592 polymorphisms or in the allele and haplotype frequencies between IBS children and HCs. PBMCs from children with IBS had significantly lower IL-10 levels after LPS stimulation than PBMCs from HCs (p=0.011); however, LPS-induced IL-10 levels in PBMCs with different genotypes of -819 and -592 polymorphisms were not significantly different between IBS patients and HCs.
conclusionsAlthough significantly lower LPS-induced IL-10 production by PBMCs was noted, it is unlikely that IL-10 production was fully genetically determined in our IBS children. ClinicalTrials.gov identifier: NCT01131442.
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Registered trials
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