ArticleThe Plant cell2013
The caspase-related protease separase (extra spindle poles) regulates cell polarity and cytokinesis in Arabidopsis.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Outer kinetochore proteins form linear elements to regulate vesicle transport.Journal of cell science · 2026Article
- Subcellular targets and recognition mechanism ofiScience · 2025Article
- Securin regulates the spatiotemporal dynamics of separase.The Journal of cell biology · 2025Article
- Centrosomes and associated proteins in pathogenesis and treatment of breast cancer.Frontiers in oncology · 2024Review
- Securin Regulates the Spatiotemporal Dynamics of Separase.bioRxiv : the preprint server for biology · 2023Article
- SEC14-like condensate phase transitions at plasma membranes regulate root growth in Arabidopsis.PLoS biology · 2023Article
- Arabidopsis metacaspase MC1 localizes in stress granules, clears protein aggregates, and delays senescence.The Plant cell · 2023Article
- The host exocyst complex is targeted by a conserved bacterial type-III effector that promotes virulence.The Plant cell · 2022Article
- Cell biology of primary cell wall synthesis in plants.The Plant cell · 2022Review
- Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis.The EMBO journal · 2021Article
- Kinetochore protein depletion underlies cytokinesis failure and somatic polyploidization in the mosseLife · 2019Article
- Conservation of the separase regulatory domain.Biology direct · 2018Article
- Genetic Identification of Separase Regulators inG3 (Bethesda, Md.) · 2018Article
- Phragmoplast microtubule dynamics - a game of zones.Journal of cell science · 2018Review
- The Metabolism of Separase Inhibitor Sepin-1 in Human, Mouse, and Rat Liver Microsomes.Frontiers in pharmacology · 2018Article
- Protease dead separase inhibits chromosome segregation and RAB-11 vesicle trafficking.Cell cycle (Georgetown, Tex.) · 2017Article
- An NADPH-Oxidase/Polyamine Oxidase Feedback Loop Controls Oxidative Burst Under Salinity.Plant physiology · 2016Article
- Tudor staphylococcal nuclease links formation of stress granules and processing bodies with mRNA catabolism in Arabidopsis.The Plant cell · 2015Article
- Tudor Staphylococcal Nuclease plays two antagonistic roles in RNA metabolism under stress.Plant signaling & behavior · 2015Article
- Wuschel-related homeobox 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce.Journal of experimental botany · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vesicle trafficking plays an important role in cell division, establishment of cell polarity, and translation of environmental cues to developmental responses. However, the molecular mechanisms regulating vesicle trafficking remain poorly understood. Here, we report that the evolutionarily conserved caspase-related protease separase (extra spindle poles [ESP]) is required for the establishment of cell polarity and cytokinesis in Arabidopsis thaliana. At the cellular level, separase colocalizes with microtubules and RabA2a (for RAS genes from rat brainA2a) GTPase-positive structures. Separase facilitates polar targeting of the auxin efflux carrier PIN-formed2 (PIN2) to the rootward side of the root cortex cells. Plants with the radially swollen4 (rsw4) allele with compromised separase activity, in addition to mitotic failure, display isotropic cell growth, perturbation of auxin gradient formation, slower gravitropic response in roots, and cytokinetic failure. Measurements of the dynamics of vesicle markers on the cell plate revealed an overall reduction of the delivery rates of KNOLLE and RabA2a GTPase in separase-deficient roots. Furthermore, dissociation of the clathrin light chain, a protein that plays major role in the formation of coated vesicles, was slower in rsw4 than in the control. Our results demonstrate that separase is a key regulator of vesicle trafficking, which is indispensable for cytokinesis and the establishment of cell polarity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.