ArticleThe Plant cell2013
The cyclophilin CYP20-2 modulates the conformation of BRASSINAZOLE-RESISTANT1, which binds the promoter of FLOWERING LOCUS D to regulate flowering in Arabidopsis.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed, 95 citations in OpenAlex.
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- Genome-wide analysis of the BES1 gene family reveals their involvement in grain development of Triticum aestivum L.BMC genomics · 2025Article
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- OsFKBP12 transduces the sucrose signal from OsNIN8 to the OsTOR pathway in a loosely binding manner for cell division.iScience · 2025Article
- A neutral invertase controls cell division besides hydrolysis of sucrose for nutrition during germination and seed setting in rice.iScience · 2024Article
- Interaction of the Transcription Factors BES1/BZR1 in Plant Growth and Stress Response.International journal of molecular sciences · 2024Review
- Transcription Factors and Their Regulatory Roles in the Male Gametophyte Development of Flowering Plants.International journal of molecular sciences · 2024Review
- The Maize ZmBES1/BZR1-9 Transcription Factor Accelerates Flowering in TransgenicPlants (Basel, Switzerland) · 2023Article
- Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns.International journal of molecular sciences · 2023Article
- Proteomic Analysis of Proteins Related to Defense Responses inInternational journal of molecular sciences · 2023Article
- Genome-wide identification and stress response analysis of cyclophilin gene family in apple (Malus × domestica).BMC genomics · 2022Article
- Comparative transcriptomics in alternate bearing cultivar Dashehari reveals the genetic model of flowering in mango.Frontiers in genetics · 2022Article
- Genome-wide detection of genotype environment interactions for flowering time inFrontiers in plant science · 2022Article
- Regulation of tomato fruit elongation by transcription factor BZR1.7 through promotion ofHorticulture research · 2022Article
- PIF4 and PIF4-Interacting Proteins: At the Nexus of Plant Light, Temperature and Hormone Signal Integrations.International journal of molecular sciences · 2021Review
- BIC1 acts as a transcriptional coactivator to promote brassinosteroid signaling and plant growth.The EMBO journal · 2021Article
- Genome-Wide Prediction, Functional Divergence, and Characterization of Stress-ResponsiveFrontiers in plant science · 2021Article
- Conserved Residues in the C-Terminal Domain Affect the Structure and Function of CYP38 in Arabidopsis.Frontiers in plant science · 2021Article
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brassinosteroids (BRs) regulate many physiological processes during plant development, including flowering. However, little is known about the components of BR signaling that mediate flowering. Here, we report that BRASSINAZOLE-RESISTANT1 (BZR1), the conformation of which is altered by a cyclophilin (CYP20-2), binds cis-elements in the FLOWERING LOCUS D (FLD) promoter to regulate flowering. Both bzr1-1D and fld-4 showed delayed flowering. Electrophoretic mobility shift assay and chromatin immunoprecipitation revealed that BZR1 bound to a putative BR response cis-element and suppressed the expression of FLD. Overexpression of FLD partially rescued the late flowering of pBZR1:mBZR1(Pro234-Leu)-CFP (mx3). Yeast two-hybrid and pull-down assays demonstrated that BZR1 interacts with CYP20-2. Arabidopsis thaliana CYP20-2 had greater peptidyl-prolyl cis-trans isomerase activity than did wheat (Triticum aestivum) CYP20-2. Fourier transform infrared spectroscopy revealed conformation changes in BZR1, dependent on interaction with CYP20-2. Due to differences in activity and substrate preference between CYP20-2 proteins from wheat and Arabidopsis, At-CYP20-2-overexpressing lines showed earlier flowering, whereas Ta CYP20-2 lines flowered later. Immunoblot and chromatin immunoprecipitation assays showed that histone H3 trimethyl Lys4 and H3 acetylation levels were negatively correlated with the transcription of FLD (a putative histone demethylase) in various lines. Therefore, a conformational change of BZR1 mediated by CYP20-2 causes altered flowering through modulation of FLD expression.
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