ArticleMicroscopy research and technique2013
Axial super resolution topography of focal adhesion by confocal microscopy.
Article in Microscopy research and technique, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles.Biophysical reports · 2023Article
- Model-free phasor image analysis of quantitative myocardial TScientific reports · 2022Article
- Regulation of IL-1 signaling through control of focal adhesion assembly.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Coordinated integrin activation by actin-dependent force during T-cell migration.Nature communications · 2016Article
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Authors and funding
2 authors.
Funding
Abstract
The protein organization within focal adhesions has been studied by state-of-the-art super resolution methods because of its thin structure, well below diffraction limit. However, to achieve high axial resolution, most of the current approaches rely on either sophisticated optics or diligent sample preparation, limiting their application. In this report we present a phasor-based method that can be applied to fluorescent samples to determine the precise axial position of proteins using a conventional confocal microscope. We demonstrate that with about 4,000 photon counts collected along a z-scan, axial localization precision close to 10 nm is achievable. We show that, with within 10 nm, the axial location of paxillin, FAK, and talin is similar at focal adhesion sites, while F-actin shows a sharp increase in height towards the cell center. We further demonstrated the live imaging capability of this method. With the advantage of simple data acquisition and no special instrument requirement, this approach could have wide dissemination and application potentials.
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Registered trials
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