ArticleInfection and immunity2013
Dissection of the role of pili and type 2 and 3 secretion systems in adherence and biofilm formation of an atypical enteropathogenic Escherichia coli strain.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Molecular Characterization of Gram-Negative Bacilli Isolated from a Neonatal Intensive Care Unit and Phenotypic and Molecular Detection of ESBL and Carbapenemase.Antibiotics (Basel, Switzerland) · 2025Article
- Antimicrobial susceptibility, biofilm formation, and virulence genes among atypical enteropathogenicIranian journal of microbiology · 2025Article
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- Genetic and Antimicrobial Resistance Profiles of Mammary PathogenicPathogens (Basel, Switzerland) · 2022Article
- Microbiology and Epidemiology ofMicroorganisms · 2022Review
- Characterization of UropathogenicMicroorganisms · 2022Article
- The Fis Nucleoid Protein Negatively Regulates the Phase VariationFrontiers in microbiology · 2022Article
- Review
- Genomic Properties and Temporal Analysis of the Interaction of an InvasiveFrontiers in cellular and infection microbiology · 2020Article
- Genetic and Virulence Characteristics of a Hybrid Atypical Enteropathogenic and UropathogenicFrontiers in cellular and infection microbiology · 2020Article
- The Type III Secretion System (T3SS)-Translocon of Atypical EnteropathogenicFrontiers in microbiology · 2019Article
- First Microbiological and Molecular Identification ofBioMed research international · 2019Article
- Article
- Expanding Role of Type II Secretion in Bacterial Pathogenesis and Beyond.Infection and immunity · 2017Review
- Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites.PloS one · 2017Article
- Diarrheagenic Escherichia coli.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2016Review
- Differential Regulation of the Surface-Exposed and Secreted SslE Lipoprotein in Extraintestinal Pathogenic Escherichia coli.PloS one · 2016Article
- Finding Regulators Associated with the Expression of the Long Polar Fimbriae in Enteropathogenic Escherichia coli.Journal of bacteriology · 2015Article
- Intra- and inter-species interactions within biofilms of important foodborne bacterial pathogens.Frontiers in microbiology · 2015Review
- Pathogenic E. coli exploits SslE mucinase activity to translocate through the mucosal barrier and get access to host cells.PloS one · 2015Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Atypical enteropathogenic Escherichia coli (aEPEC) strains are diarrheal pathogens that lack bundle-forming pilus production but possess the virulence-associated locus of enterocyte effacement. aEPEC strain 1551-2 produces localized adherence (LA) on HeLa cells; however, its isogenic intimin (eae) mutant produces a diffuse-adherence (DA) pattern. In this study, we aimed to identify the DA-associated adhesin of the 1551-2 eae mutant. Electron microscopy of 1551-2 identified rigid rod-like pili composed of an 18-kDa protein, which was identified as the major pilin subunit of type 1 pilus (T1P) by mass spectrometry analysis. Deletion of fimA in 1551-2 affected biofilm formation but had no effect on adherence properties. Analysis of secreted proteins in supernatants of this strain identified a 150-kDa protein corresponding to SslE, a type 2 secreted protein that was recently reported to be involved in biofilm formation of rabbit and human EPEC strains. However, neither adherence nor biofilm formation was affected in a 1551-2 sslE mutant. We then investigated the role of the EspA filament associated with the type 3 secretion system (T3SS) in DA by generating a double eae espA mutant. This strain was no longer adherent, strongly suggesting that the T3SS translocon is the DA adhesin. In agreement with these results, specific anti-EspA antibodies blocked adherence of the 1551-2 eae mutant. Our data support a role for intimin in LA, for the T3SS translocon in DA, and for T1P in biofilm formation, all of which may act in concert to facilitate host intestinal colonization by aEPEC strains.
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