ArticleAlcoholism, clinical and experimental research2013
Zinc-dependent modulation of α2- and α3-glycine receptor subunits by ethanol.
Article in Alcoholism, clinical and experimental research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Methods for negating the impact of zinc contamination to allow characterization of positive allosteric modulators of glycine receptors.Frontiers in molecular neuroscience · 2024Article
- Positive Allosteric Modulators of Glycine Receptors and Their Potential Use in Pain Therapies.Pharmacological reviews · 2022Review
- Modulatory Actions of the Glycine Receptor β Subunit on the Positive Allosteric Modulation of Ethanol in α2 Containing Receptors.Frontiers in molecular neuroscience · 2021Article
- SNARE Complex-Associated Proteins and Alcohol.Alcoholism, clinical and experimental research · 2020Review
- Glycine receptor α3 and α2 subunits mediate tonic and exogenous agonist-induced currents in forebrain.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Recreational concentrations of alcohol enhance synaptic inhibition of cerebellar unipolar brush cells via pre- and postsynaptic mechanisms.Journal of neurophysiology · 2017Article
- Interactions between Zinc and Allosteric Modulators of the Glycine Receptor.The Journal of pharmacology and experimental therapeutics · 2017Article
- Identification and characterization of heptapeptide modulators of the glycine receptor.European journal of pharmacology · 2016Article
- Identification of Parvalbumin Interneurons as Cellular Substrate of Fear Memory Persistence.Cerebral cortex (New York, N.Y. : 1991) · 2016Article
- Defects of the Glycinergic Synapse in Zebrafish.Frontiers in molecular neuroscience · 2016Review
- Ethanol effects on glycinergic transmission: From molecular pharmacology to behavior responses.Pharmacological research · 2015Review
- Allosteric modulation of the glycine receptor activated by agonists differing in efficacy.Brain research · 2015Article
- Glycine receptors containing α2 or α3 subunits regulate specific ethanol-mediated behaviors.The Journal of pharmacology and experimental therapeutics · 2015Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundStrychnine-sensitive glycine receptors (GlyRs) are expressed throughout the brain and spinal cord and are among the strongly supported protein targets of alcohol. This is based largely on studies of the α1-subunit; however, α2- and α3-GlyR subunits are as or more abundantly expressed than α1-GlyRs in multiple forebrain brain areas considered to be important for alcohol-related behaviors, and uniquely some α3-GlyRs undergo RNA editing. Nanomolar and low micromolar concentrations of zinc ions potentiate GlyR function, and in addition to zinc's effects on glycine-activated currents, we have recently shown that physiological concentrations of zinc also enhance the magnitude of ethanol (EtOH)'s effects on α1-GlyRs.
methodsUsing 2-electrode voltage-clamp electrophysiology in oocytes expressing either α2- or α3-GlyRs, we first tested the hypothesis that the effects of EtOH on α2- and α3-GlyRs would be zinc dependent, as we have previously reported for α1-GlyRs. Next, we constructed an α3P185L-mutant GlyR to test whether RNA-edited and unedited GlyRs contain differences in EtOH sensitivity. Last, we built a homology model of the α3-GlyR subunit.
resultsThe effects of EtOH (20 to 200 mM) on both subunits were greater in the presence than in the absence of 500 nM added zinc. The α3P185L-mutation that corresponds to RNA editing increased sensitivity to glycine and decreased sensitivity to EtOH.
conclusionsOur findings provide further evidence that zinc is important for determining the magnitude of EtOH's effects at GlyRs and suggest that by better understanding zinc/EtOH interactions at GlyRs, we may better understand the sites and mechanisms of EtOH action.
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