ArticlePloS one2013
Proteome analysis and serological characterization of surface-exposed proteins of Rickettsia heilongjiangensis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Bridging historical antigenic profiling and whole-genome taxonomy inScientific reviews · 2026Review
- Vaccine development: obligate intracellular bacteria new tools, old pathogens: the current state of vaccines against obligate intracellular bacteria.Frontiers in cellular and infection microbiology · 2024Review
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- The neglected challenge: Vaccination against rickettsiae.PLoS neglected tropical diseases · 2020Review
- Proteomic analysis of Rickettsia akari proposes a 44 kDa-OMP as a potential biomarker for Rickettsialpox diagnosis.BMC microbiology · 2020Article
- Identification of a Ribosomal Protein RpsB as a Surface-Exposed Protein and Adhesin ofBioMed research international · 2019Article
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- Rickettsia Lipid A Biosynthesis Utilizes the Late Acyltransferase LpxJ for Secondary Fatty Acid Addition.Journal of bacteriology · 2018Article
- Multi-omics Analysis Sheds Light on the Evolution and the Intracellular Lifestyle Strategies of Spotted Fever GroupFrontiers in microbiology · 2017Article
- Immunoproteomic profiling of Rickettsia parkeri and Rickettsia amblyommii.Ticks and tick-borne diseases · 2015Article
- Misdiagnosed murine typhus in a patient with multiple cerebral infarctions and hemorrhage: a case report.BMC neurology · 2015Article
- Secretome of obligate intracellular Rickettsia.FEMS microbiology reviews · 2015Review
- Rickettsia rickettsii outer membrane protein YbgF induces protective immunity in C3H/HeN mice.Human vaccines & immunotherapeutics · 2015Article
- Article
- Microarray of surface-exposed proteins of Rickettsia heilongjiangensis for serodiagnosis of Far-eastern spotted fever.BMC infectious diseases · 2014Article
- Identification of novel surface-exposed proteins of Rickettsia rickettsii by affinity purification and proteomics.PloS one · 2014Article
- Article
- Diversity of Rickettsiales in the microbiome of the lone star tick, Amblyomma americanum.Applied and environmental microbiology · 2014Article
- Exploratory study on Th1 epitope-induced protective immunity against Coxiella burnetii infection.PloS one · 2014Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRickettsia heilongjiangensis, the agent of Far-Eastern spotted fever (FESF), is an obligate intracellular bacterium. The surface-exposed proteins (SEPs) of rickettsiae are involved in rickettsial adherence to and invasion of host cells, intracellular bacterial growth, and/or interaction with immune cells. They are also potential molecular candidates for the development of diagnostic reagents and vaccines against rickettsiosis.
methodsR. heilongjiangensis SEPs were identified by biotin-streptavidin affinity purification and 2D electrophoreses coupled with ESI-MS/MS. Recombinant SEPs were probed with various sera to analyze their serological characteristics using a protein microarray and an enzyme-linked immune sorbent assay (ELISA).
resultsTwenty-five SEPs were identified, most of which were predicted to reside on the surface of R. heilongjiangensis cells. Bioinformatics analysis suggests that these proteins could be involved in bacterial pathogenesis. Eleven of the 25 SEPs were recognized as major seroreactive antigens by sera from R. heilongjiangensis-infected mice and FESF patients. Among the major seroreactive SEPs, microarray assays and/or ELISAs revealed that GroEL, OmpA-2, OmpB-3, PrsA, RplY, RpsB, SurA and YbgF had modest sensitivity and specificity for recognizing R. heilongjiangensis infection and/or spotted fever.
conclusionsMany of the SEPs identified herein have potentially important roles in R. heilongjiangensis pathogenicity. Some of them have potential as serodiagnostic antigens or as subunit vaccine antigens against the disease.
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