ArticlePloS one2013
RNAi-directed downregulation of vacuolar H(+) -ATPase subunit a results in enhanced stomatal aperture and density in rice.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 42 citations in OpenAlex.
- V-ATPases and amino acid catabolism are strongly disturbed in the roots of autophagy mutants in Arabidopsis, impacting water use efficiency and tricarboxylic acid metabolism.The Plant journal : for cell and molecular biology · 2025Article
- Identification of candidate genes for drought tolerance in soybean through QTL mapping and gene expression analysis.Frontiers in genetics · 2025Article
- Arabidopsis COP1 guides stomatal response in guard cells through pH regulation.Communications biology · 2024Article
- Cytosolic alkalinization in guard cells: an intriguing but interesting event during stomatal closure that merits further validation of its importance.Frontiers in plant science · 2024Review
- Regulation of V-ATPase by Jasmonic Acid: Possible Role of Persulfidation.International journal of molecular sciences · 2023Article
- Genome assembly and genetic dissection of a prominent drought-resistant maize germplasm.Nature genetics · 2023Article
- The Plant V-ATPase.Frontiers in plant science · 2022Review
- RNA-seq analysis reveals genes related to photosynthetic carbon partitioning and lipid production inFrontiers in microbiology · 2022Article
- Molecular insights into plant desiccation tolerance: transcriptomics, proteomics and targeted metabolite profiling in Craterostigma plantagineum.The Plant journal : for cell and molecular biology · 2021Article
- Physiological and Transcriptome Analyses of Early Leaf Senescence forInternational journal of molecular sciences · 2019Article
- Gene mutation associated with esl mediates shifts on fungal community composition in rhizosphere soil of rice at grain-filling stage.Scientific reports · 2018Article
- The Gene Encoding Subunit A of the Vacuolar HFrontiers in plant science · 2018Article
- The V-ATPase subunit A is essential for salt tolerance through participating in vacuolar NaPlanta · 2017Article
- Suppression of OsVPE3 Enhances Salt Tolerance by Attenuating Vacuole Rupture during Programmed Cell Death and Affects Stomata Development in Rice.Rice (New York, N.Y.) · 2016Article
- A pivotal role of vacuolar H(+)-ATPase in regulation of lipid production in Phaeodactylum tricornutum.Scientific reports · 2016Article
- Ascribing Functions to Genes: Journey Towards Genetic Improvement of Rice Via Functional Genomics.Current genomics · 2016Article
- A single cytosine deletion in the OsPLS1 gene encoding vacuolar-type H+-ATPase subunit A1 leads to premature leaf senescence and seed dormancy in rice.Journal of experimental botany · 2016Article
- Phosphatidylinositol 3-Kinase Promotes Activation and Vacuolar Acidification and Delays Methyl Jasmonate-Induced Leaf Senescence.Plant physiology · 2016Article
- MAPK-mediated enhanced expression of vacuolar H(+)-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint (Mentha piperita L.).Protoplasma · 2016Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stomatal movement plays a key role in plant development and response to drought and salt stress by regulating gas exchange and water loss. A number of genes have been demonstrated to be involved in the regulation of this process. Using inverse genetics approach, we characterized the function of a rice (Oryza sativa L.) vacuolar H(+)-ATPase subunit A (OsVHA-A) gene in stomatal conductance regulation and physiological response to salt and osmotic stress. OsVHA-A was constitutively expressed in different rice tissues, and the fusion protein of GFP-OsVHA-A was exclusively targeted to tonoplast when transiently expressed in the onion epidermal cells. Heterologous expression of OsVHA-A was able to rescue the yeast mutant vma1Δ (lacking subunit A activity) phenotype, suggesting that it partially restores the activity of V-ATPase. Meanwhile, RNAi-directed knockdown of OsVHA-A led to a reduction of vacuolar H(+)-ATPase activity and an enhancement of plasma membrane H(+)-ATPase activity, thereby increasing the concentrations of extracellular H(+) and intracellular K(+) and Na(+) under stress conditions. Knockdown of OsVHA-A also resulted in the upregulation of PAM3 (plasma membrane H(+)-ATPase 3) and downregulation of CAM1 (calmodulin 1), CAM3 (calmodulin 3) and YDA1 (YODA, a MAPKK gene). Altered level of the ion concentration and the gene expression by knockdown of OsVHA-A probably resulted in expanded aperture of stomatal pores and increased stomatal density. In addition, OsVHA-A RNAi plants displayed significant growth inhibition under salt and osmotic stress conditions. Taken together, our results suggest that OsVHA-A takes part in regulating stomatal density and opening via interfering with pH value and ionic equilibrium in guard cells and thereby affects the growth of rice plants.
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