ArticlePloS one2013
A microfluidic DNA library preparation platform for next-generation sequencing.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Nucleic acid amplification tests in digital microfluidics: the promise of next-generation point-of-care diagnostics.Microsystems & nanoengineering · 2025Review
- Next-generation sequencing applications in food science: fundamentals and recent advances.Frontiers in bioengineering and biotechnology · 2025Review
- Automation of customizable library preparation for next-generation sequencing into an open microfluidic platform.Scientific reports · 2024Article
- Leveraging the fundamentals of heat transfer and fluid mechanics in microscale geometries for automated next-generation sequencing library preparation.Scientific reports · 2024Article
- Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification: Design, Fabrication and Characterization.Sensors (Basel, Switzerland) · 2023Article
- Faster, better, and cheaper: harnessing microfluidics and mass spectrometry for biotechnology.RSC chemical biology · 2021Review
- Drop-to-drop liquid-liquid extraction of DNA in an electrowetting-on-dielectric digital microfluidics.Biomicrofluidics · 2021Article
- Integrated magneto-electrophoresis microfluidic chip purification on library preparation device for preimplantation genetic testing for aneuploidy detection.RSC advances · 2021Article
- Advantages and Limitations of 16S rRNA Next-Generation Sequencing for Pathogen Identification in the Diagnostic Microbiology Laboratory: Perspectives from a Middle-Income Country.Diagnostics (Basel, Switzerland) · 2020Review
- Microfluidic Technologies for cfDNA Isolation and Analysis.Micromachines · 2019Review
- Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Digital Microfluidics for Nucleic Acid Amplification.Sensors (Basel, Switzerland) · 2017Review
- Microfluidics for genome-wide studies involving next generation sequencing.Biomicrofluidics · 2017Review
- High-throughput automated microfluidic sample preparation for accurate microbial genomics.Nature communications · 2017Article
- Evaluation of library preparation methods for Illumina next generation sequencing of small amounts of DNA from foodborne parasites.Journal of microbiological methods · 2016Article
- A parallelized microfluidic DNA bisulfite conversion module for streamlined methylation analysis.Biomedical microdevices · 2016Article
- Genome-wide epigenomic profiling for biomarker discovery.Clinical epigenetics · 2016Review
- Article
- Resistance determinants and mobile genetic elements of an NDM-1-encoding Klebsiella pneumoniae strain.PloS one · 2014Article
- Automated long-term monitoring of parallel microfluidic operations applying a machine vision-assisted positioning method.TheScientificWorldJournal · 2014Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Next-generation sequencing (NGS) is emerging as a powerful tool for elucidating genetic information for a wide range of applications. Unfortunately, the surging popularity of NGS has not yet been accompanied by an improvement in automated techniques for preparing formatted sequencing libraries. To address this challenge, we have developed a prototype microfluidic system for preparing sequencer-ready DNA libraries for analysis by Illumina sequencing. Our system combines droplet-based digital microfluidic (DMF) sample handling with peripheral modules to create a fully-integrated, sample-in library-out platform. In this report, we use our automated system to prepare NGS libraries from samples of human and bacterial genomic DNA. E. coli libraries prepared on-device from 5 ng of total DNA yielded excellent sequence coverage over the entire bacterial genome, with >99% alignment to the reference genome, even genome coverage, and good quality scores. Furthermore, we produced a de novo assembly on a previously unsequenced multi-drug resistant Klebsiella pneumoniae strain BAA-2146 (KpnNDM). The new method described here is fast, robust, scalable, and automated. Our device for library preparation will assist in the integration of NGS technology into a wide variety of laboratories, including small research laboratories and clinical laboratories.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.