ArticlePloS one2013
miR-27b represses migration of mouse MSCs to burned margins and prolongs wound repair through silencing SDF-1a.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- The Wound-Heart Axis: Can Chronic Wounds Contribute to Cardiac Dysfunction?International journal of molecular sciences · 2026Review
- Therapeutic Potential of Modulating Gene-MicroRNA Crosstalk in Burn Injury.International journal of molecular sciences · 2025Review
- Exosomes and microRNAs: insights into their roles in thermal-induced skin injury, wound healing and scarring.Molecular genetics and genomics : MGG · 2024Review
- MSCs and their exosomes: a rapidly evolving approach in the context of cutaneous wounds therapy.Stem cell research & therapy · 2021Review
- mmu-miR-145a-5p Accelerates Diabetic Wound Healing by Promoting Macrophage Polarization Toward the M2 Phenotype.Frontiers in medicine · 2021Article
- Mesenchymal stem cell-based therapy for burn wound healing.Burns & trauma · 2021Review
- Extracellular vesicle-carried microRNA-27b derived from mesenchymal stem cells accelerates cutaneous wound healing via E3 ubiquitin ligase ITCH.Journal of cellular and molecular medicine · 2020Article
- miR-27b promotes angiogenesis and skin repair in scalded rats through regulating VEGF-C expression.Lasers in medical science · 2020Article
- MiR-27b-3p promotes migration and invasion in colorectal cancer cells by targeting HOXA10.Bioscience reports · 2019Article
- The role of microRNAs in the healing of diabetic ulcers.International wound journal · 2019Review
- Therapeutic strategies for enhancing angiogenesis in wound healing.Advanced drug delivery reviews · 2019Review
- MicroRNAs in diabetic wound healing: Pathophysiology and therapeutic opportunities.Trends in cardiovascular medicine · 2019Review
- MicroRNAs in the Migration of Mesenchymal Stem Cells.Stem cell reviews and reports · 2019Review
- MiR-539-5p negatively regulates migration of rMSCs induced by Bushen Huoxue decoction through targeting Wnt5a.International journal of medical sciences · 2019Article
- miR-27a-containing Exosomes Secreted by Irradiated Skin Keratinocytes Delayed the Migration of Unirradiated Skin Fibroblasts.International journal of biological sciences · 2019Article
- Transition from inflammation to proliferation: a critical step during wound healing.Cellular and molecular life sciences : CMLS · 2016Review
- Article
- Simvastatin improves the homing of BMSCs via the PI3K/AKT/miR-9 pathway.Journal of cellular and molecular medicine · 2016Article
- CXCL12/CXCR4: a symbiotic bridge linking cancer cells and their stromal neighbors in oncogenic communication networks.Oncogene · 2016Review
- Protective role of microRNA-29a in denatured dermis and skin fibroblast cells after thermal injury.Biology open · 2016Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInteractions between stromal cell-derived factor-1α (SDF-1α) and its cognate receptor CXCR4 are crucial for the recruitment of mesenchymal stem cells (MSCs) from bone marrow (BM) reservoirs to damaged tissues for repair during alarm situations. MicroRNAs are differentially expressed in stem cell niches, suggesting a specialized role in stem cell regulation. Here, we gain insight into the molecular mechanisms involved in regulating SDF-1α.
methodsMSCs from green fluorescent protein transgenic male mice were transfused to irradiated recipient female C57BL/6 mice, and skin burn model of bone marrow-chimeric mice were constructed. Six miRNAs with differential expression in burned murine skin tissue compared to normal skin tissue were identified using microarrays and bioinformatics. The expression of miR-27b and SDF-1α was examined in burned murine skin tissue using quantitative real-time PCR (qPCR) and immunohistochemistry (IHC), enzyme-linked immunosorbent assay (ELISA). The Correlation of miR-27b and SDF-1α expression was analyzed by Pearson analysis Correlation. miRNAs suppressed SDF-1α protein expression by binding directly to its 3'UTR using western blot and luciferase reporter assay. The importance of miRNAs in MSCs chemotaxis was further estimated by decreasing SDF-1α in vivo and in vitro.
resultsmiR-23a, miR-27a and miR-27b expression was significantly lower in the burned skin than in the normal skin (p<0.05). We also found that several miRNAs suppressed SDF-1α protein expression, while just miR-27a and miR-27b directly bound to the SDF-1α 3'UTR. Moreover, the forced over-expression of miR-27a and miR-27b significantly reduced the directional migration of mMSCs in vitro. However, only miR-27b in burn wound margins significantly inhibited the mobilization of MSCs to the epidermis.
conclusionmiR-27b may be a unique signature of the stem cell niche in burned mouse skin and can suppress the directional migration of mMSCs by targeting SDF-1α by binding directly to its 3'UTR.
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