ArticlePloS one2013
The transcriptomic signature of RacA activation and inactivation provides new insights into the morphogenetic network of Aspergillus niger.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Oxygen Consumption in Filamentous Pellets of Aspergillus niger: Microelectrode Measurements and Modeling.Biotechnology and bioengineering · 2025Article
- Evolution and screening of Trichoderma reesei mutants for secreted protein production at elevated temperature.Journal of industrial microbiology & biotechnology · 2024Article
- Contrasting effects of NADPH oxidases on the fungal hyphae growth and immune responses inFrontiers in microbiology · 2024Article
- Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability.Materials today. Bio · 2023Article
- GTPase Rac Regulates Conidiation, AFB1 Production and Stress Response in Pathogenic FungusToxins · 2022Article
- Effect ofACS omega · 2022Article
- Turning Inside Out: Filamentous Fungal Secretion and Its Applications in Biotechnology, Agriculture, and the Clinic.Journal of fungi (Basel, Switzerland) · 2021Review
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- Reduced viscosity mutants of Trichoderma reesei with improved industrial fermentation characteristics.Journal of industrial microbiology & biotechnology · 2021Article
- Genetic Characterization of Mutations Related to Conidiophore Stalk Length Development inFrontiers in genetics · 2021Article
- Article
- Harnessing the knowledge of protein secretion for enhanced protein production in filamentous fungi.World journal of microbiology & biotechnology · 2019Review
- Systems metabolic engineering for citric acid production by Aspergillus niger in the post-genomic era.Microbial cell factories · 2019Review
- Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi.Fungal biology and biotechnology · 2019Article
- Moulding the mould: understanding and reprogramming filamentous fungal growth and morphogenesis for next generation cell factories.Biotechnology for biofuels · 2019Review
- Deletion of the small GTPaseFungal biology and biotechnology · 2019Article
- Farnesol-induced hyperbranched morphology with short hyphae and bulbous tips of Coriolus versicolor.Scientific reports · 2018Article
- Construction of an improved Aspergillus niger platform for enhanced glucoamylase secretion.Microbial cell factories · 2018Article
- How a fungus shapes biotechnology: 100 years ofFungal biology and biotechnology · 2018Review
- Conditional Expression of the Small GTPase ArfA Impacts Secretion, Morphology, Growth, and Actin Ring Position inFrontiers in microbiology · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RacA is the main Rho GTPase in Aspergillus niger regulating polarity maintenance via controlling actin dynamics. Both deletion and dominant activation of RacA (Rac(G18V)) provoke an actin localization defect and thereby loss of polarized tip extension, resulting in frequent dichotomous branching in the ΔracA strain and an apolar growing phenotype for Rac(G18V). In the current study the transcriptomics and physiological consequences of these morphological changes were investigated and compared with the data of the morphogenetic network model for the dichotomous branching mutant ramosa-1. This integrated approach revealed that polar tip growth is most likely orchestrated by the concerted activities of phospholipid signaling, sphingolipid signaling, TORC2 signaling, calcium signaling and CWI signaling pathways. The transcriptomic signatures and the reconstructed network model for all three morphology mutants (ΔracA, Rac(G18V), ramosa-1) imply that these pathways become integrated to bring about different physiological adaptations including changes in sterol, zinc and amino acid metabolism and changes in ion transport and protein trafficking. Finally, the fate of exocytotic (SncA) and endocytotic (AbpA, SlaB) markers in the dichotomous branching mutant ΔracA was followed, demonstrating that hyperbranching does not per se result in increased protein secretion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.