ArticleRNA (New York, N.Y.)2013
An RpoS-dependent sRNA regulates the expression of a chaperone involved in protein folding.
Article in RNA (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- FinO/ProQ-family proteins: an evolutionary perspective.Bioscience reports · 2023Review
- A paradox of bacterial persistence and antibiotic resistance: chloramphenicol acetyl transferase as a double barrel shot gun.microLife · 2023Article
- In vivo targets of Salmonella FinO include a FinP-like small RNA controlling copy number of a cohabitating plasmid.Nucleic acids research · 2021Article
- Article
- SraL sRNA interaction regulates the terminator by preventing premature transcription termination ofProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Functional Transcriptomics for Bacterial Gene Detectives.Microbiology spectrum · 2018Review
- Small Regulatory RNAs in the Enterobacterial Response to Envelope Damage and Oxidative Stress.Microbiology spectrum · 2018Review
- Bacterial Small RNAs in Mixed Regulatory Networks.Microbiology spectrum · 2018Review
- Gene network analysis identifies a central post-transcriptional regulator of cellular stress survival.eLife · 2018Article
- RNA search engines empower the bacterial intranet.Biochemical Society transactions · 2017Review
- Proteome remodelling by the stress sigma factor RpoS/σScientific reports · 2017Article
- Small regulatory bacterial RNAs regulating the envelope stress response.Biochemical Society transactions · 2017Review
- Molecular mechanism of mRNA repression inThe EMBO journal · 2017Article
- The target spectrum of SdsR small RNA in Salmonella.Nucleic acids research · 2016Article
- Grad-seq guides the discovery of ProQ as a major small RNA-binding protein.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Protection against deleterious nitrogen compounds: role of σS-dependent small RNAs encoded adjacent to sdiA.Nucleic acids research · 2016Article
- The Impact of 18 Ancestral and Horizontally-Acquired Regulatory Proteins upon the Transcriptome and sRNA Landscape of Salmonella enterica serovar Typhimurium.PLoS genetics · 2016Article
- Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli.BMC genomics · 2015Article
- Repressor activity of the RpoS/σS-dependent RNA polymerase requires DNA binding.Nucleic acids research · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small noncoding RNAs (sRNAs) are usually expressed in the cell to face a variety of stresses. In this report we disclose the first target for SraL (also known as RyjA), a sRNA present in many bacteria, which is highly induced in stationary phase. We also demonstrate that this sRNA is directly transcribed by the major stress σ factor σ(S) (RpoS) in Salmonella enterica serovar Typhimurium. We show that SraL sRNA down-regulates the expression of the chaperone Trigger Factor (TF), encoded by the tig gene. TF is one of the three major chaperones that cooperate in the folding of the newly synthesized cytosolic proteins and is the only ribosome-associated chaperone known in bacteria. By use of bioinformatic tools and mutagenesis experiments, SraL was shown to directly interact with the 5' UTR of the tig mRNA a few nucleotides upstream of the Shine-Dalgarno region. Namely, point mutations in the sRNA (SraL*) abolished the repression of tig mRNA and could only down-regulate a tig transcript target with the respective compensatory mutations. We have also validated in vitro that SraL forms a stable duplex with the tig mRNA. This work constitutes the first report of a small RNA affecting protein folding. Taking into account that both SraL and TF are very well conserved in enterobacteria, this work will have important repercussions in the field.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.