ReviewJournal of cellular physiology2014
Bending the rules: widefield microscopy and the Abbe limit of resolution.
Review in Journal of cellular physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Translocation of (ultra)fine particles and nanoparticles across the placenta; a systematic review on the evidence of in vitro, ex vivo, and in vivo studies.Particle and fibre toxicology · 2020Pooled it
- High-resolution multi-modal imaging of sub-cellular structures with low numerical aperture objective.JPhys photonics · 2025Article
- A method for reproducible high-resolution imaging of 3D cancer cell spheroids.Journal of microscopy · 2023Article
- A biologist's guide to planning and performing quantitative bioimaging experiments.PLoS biology · 2023Article
- MicroLED/LED electro-optical integration techniques for non-display applications.Applied physics reviews · 2023Review
- What Is Motion? Recent Advances in the Study of Molecular Movement Patterns of the Peptidoglycan Synthesis Machines.Journal of bacteriology · 2022Review
- Compartmentalization of Sphingolipid metabolism: Implications for signaling and therapy.Pharmacology & therapeutics · 2022Review
- A Layered View on Focal Adhesions.Biology · 2021Review
- The frontier of live tissue imaging across space and time.Cell stem cell · 2021Review
- The construction of high-magnification homemade lenses for a simple microscope: an easy "DIY" tool for biological and interdisciplinary education.Advances in physiology education · 2021Article
- A comparative study on the use of microscopy in pharmacology and cell biology research.PloS one · 2021Article
- From Microscopy to Nanoscopy: Defining anFrontiers in plant science · 2021Review
- Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications.Genetics · 2019Review
- Geometric partitioning of cohesin and condensin is a consequence of chromatin loops.Molecular biology of the cell · 2018Article
- Methods for imaging mammalian mitochondrial morphology: A prospective on MitoGraph.Analytical biochemistry · 2018Article
- Navigating challenges in the application of superresolution microscopy.The Journal of cell biology · 2017Review
- Visualization of the 3D structures of small organisms via LED-SIM.Frontiers in zoology · 2016Article
- Superresolution live imaging of plant cells using structured illumination microscopy.Nature protocols · 2015Article
- Dynamics and organization of cortical microtubules as revealed by superresolution structured illumination microscopy.Plant physiology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
One of the most fundamental concepts of microscopy is that of resolution-the ability to clearly distinguish two objects as separate. Recent advances such as structured illumination microscopy (SIM) and point localization techniques including photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM) strive to overcome the inherent limits of resolution of the modern light microscope. These techniques, however, are not always feasible or optimal for live cell imaging. Thus, in this review, we explore three techniques for extracting high resolution data from images acquired on a widefield microscope-deconvolution, model convolution, and Gaussian fitting. Deconvolution is a powerful tool for restoring a blurred image using knowledge of the point spread function (PSF) describing the blurring of light by the microscope, although care must be taken to ensure accuracy of subsequent quantitative analysis. The process of model convolution also requires knowledge of the PSF to blur a simulated image which can then be compared to the experimentally acquired data to reach conclusions regarding its geometry and fluorophore distribution. Gaussian fitting is the basis for point localization microscopy, and can also be applied to tracking spot motion over time or measuring spot shape and size. All together, these three methods serve as powerful tools for high-resolution imaging using widefield microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.