ArticleGenetics2013
Integration profiling of gene function with dense maps of transposon integration.
Article in Genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 72 citations in OpenAlex.
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- The power of many: when genetics met yeasts and high-throughput.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Facile generation ofMicrobiology spectrum · 2026Article
- Landscape of essential growth and fluconazole-resistance genes in the human fungal pathogen Cryptococcus neoformans.PLoS biology · 2025Article
- Article
- Essential Genes Discovery in Microorganisms by Transposon-Directed Sequencing (Tn-Seq): Experimental Approaches, Major Goals, and Future Perspectives.International journal of molecular sciences · 2024Review
- Copy number variation alters local and global mutational tolerance.Genome research · 2023Article
- Nucleotide exchange is sufficient for Hsp90 functions in vivo.Nature communications · 2023Article
- Genome-wide quantification of contributions to sexual fitness identifies genes required for spore viability and health in fission yeast.PLoS genetics · 2022Article
- A multiplexed, three-dimensional pooling and next-generation sequencing strategy for creating barcoded mutant arrays: construction of a Schizosaccharomyces pombe transposon insertion library.Nucleic acids research · 2022Article
- Engineered Sleeping Beauty transposase redirects transposon integration away from genes.Nucleic acids research · 2022Article
- Genome-wide screens in yeast models towards understanding chronological lifespan regulation.Briefings in functional genomics · 2022Article
- SAturated Transposon Analysis in Yeast (SATAY) for Deep Functional Mapping of Yeast Genomes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Identifying essential genes across eukaryotes by machine learning.NAR genomics and bioinformatics · 2021Article
- A protocol for transposon insertion sequencing inSTAR protocols · 2021Article
- Article
- Hijacking Transposable Elements for Saturation Mutagenesis in Fungi.Frontiers in fungal biology · 2021Review
- The Integration Preference of Sleeping Beauty at Non-TA Site Is Related to the Transposon End Sequences.Frontiers in genetics · 2021Article
- Comparing the utility of in vivo transposon mutagenesis approaches in yeast species to infer gene essentiality.Current genetics · 2020Article
- Transposon Insertion Sequencing, a Global Measure of Gene Function.Annual review of genetics · 2020Review
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Authors and funding
11 authors at 6 institutions in 2 countries.
Funding
Abstract
Understanding how complex networks of genes integrate to produce dividing cells is an important goal that is limited by the difficulty in defining the function of individual genes. Current resources for the systematic identification of gene function such as siRNA libraries and collections of deletion strains are costly and organism specific. We describe here integration profiling, a novel approach to identify the function of eukaryotic genes based upon dense maps of transposon integration. As a proof of concept, we used the transposon Hermes to generate a library of 360,513 insertions in the genome of Schizosaccharomyces pombe. On average, we obtained one insertion for every 29 bp of the genome. Hermes integrated more often into nucleosome free sites and 33% of the insertions occurred in ORFs. We found that ORFs with low integration densities successfully identified the genes that are essential for cell division. Importantly, the nonessential ORFs with intermediate levels of insertion correlated with the nonessential genes that have functions required for colonies to reach full size. This finding indicates that integration profiles can measure the contribution of nonessential genes to cell division. While integration profiling succeeded in identifying genes necessary for propagation, it also has the potential to identify genes important for many other functions such as DNA repair, stress response, and meiosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.