ArticleGenetics2013
Forward genetics by genome sequencing reveals that rapid cyanide release deters insect herbivory of Sorghum bicolor.
Article in Genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- Discovery of a defence-response gene forFrontiers in plant science · 2026Article
- Estimating sorghum leaf dhurrin content using a handheld near infrared instrument.Frontiers in plant science · 2026Article
- Directed mutagenesis in fruit crops.3 Biotech · 2025Review
- Dhurrin: a potential endogenous nitrogen turnover source for early seedling growth in sorghum.Frontiers in plant science · 2025Article
- A Review of Recent Advances in Chromatographic Quantification Methods for Cyanogenic Glycosides.Molecules (Basel, Switzerland) · 2024Review
- Dhurrin in Sorghum: Biosynthesis, Regulation, Biological Function and Challenges for Animal Production.Plants (Basel, Switzerland) · 2024Review
- Cyanogenesis, a Plant Defence Strategy against Herbivores.International journal of molecular sciences · 2023Review
- Genetic variation in hydrogen cyanide potential of perennial sorghum evaluated by colorimetry.Plant direct · 2022Article
- Seedling growth and fall armyworm feeding preference influenced by dhurrin production in sorghum.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Article
- Article
- The Sorghum (Frontiers in plant science · 2021Article
- Allocation of Resources to Cyanogenic Glucosides Does Not Incur a Growth Sacrifice inPlants (Basel, Switzerland) · 2020Article
- Prediction of Dhurrin Metabolism by Transcriptome and Metabolome Analyses inPlants (Basel, Switzerland) · 2020Article
- Mutations in sorghum SBEIIb and SSIIa affect alkali spreading value, starch composition, thermal properties and flour viscosity.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Whole-Genome Sequence Accuracy Is Improved by Replication in a Population of Mutagenized Sorghum.G3 (Bethesda, Md.) · 2018Article
- The first identification of genomic loci in plants associated with resistance to galling insects: a case study in Eucalyptus L'Hér. (Myrtaceae).Scientific reports · 2018Article
- Forward Genetics by Sequencing EMS Variation-Induced Inbred Lines.G3 (Bethesda, Md.) · 2017Article
- A Sorghum Mutant Resource as an Efficient Platform for Gene Discovery in Grasses.The Plant cell · 2016Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whole genome sequencing has allowed rapid progress in the application of forward genetics in model species. In this study, we demonstrated an application of next-generation sequencing for forward genetics in a complex crop genome. We sequenced an ethyl methanesulfonate-induced mutant of Sorghum bicolor defective in hydrogen cyanide release and identified the causal mutation. A workflow identified the causal polymorphism relative to the reference BTx623 genome by integrating data from single nucleotide polymorphism identification, prior information about candidate gene(s) implicated in cyanogenesis, mutation spectra, and polymorphisms likely to affect phenotypic changes. A point mutation resulting in a premature stop codon in the coding sequence of dhurrinase2, which encodes a protein involved in the dhurrin catabolic pathway, was responsible for the acyanogenic phenotype. Cyanogenic glucosides are not cyanogenic compounds but their cyanohydrins derivatives do release cyanide. The mutant accumulated the glucoside, dhurrin, but failed to efficiently release cyanide upon tissue disruption. Thus, we tested the effects of cyanide release on insect herbivory in a genetic background in which accumulation of cyanogenic glucoside is unchanged. Insect preference choice experiments and herbivory measurements demonstrate a deterrent effect of cyanide release capacity, even in the presence of wild-type levels of cyanogenic glucoside accumulation. Our gene cloning method substantiates the value of (1) a sequenced genome, (2) a strongly penetrant and easily measurable phenotype, and (3) a workflow to pinpoint a causal mutation in crop genomes and accelerate in the discovery of gene function in the postgenomic era.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.