ArticleJournal of neuroscience research2013
Process-based expansion and neural differentiation of human pluripotent stem cells for transplantation and disease modeling.
Article in Journal of neuroscience research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- The Autism Spectrum Disorders Stem Cell Resource at Children's Hospital of Orange County: Implications for Disease Modeling and Drug Discovery.Stem cells translational medicine · 2014Trial
- Human iPSC-derived neural stem cells engraft and improve pathophysiology of MPS I mice.Molecular therapy. Methods & clinical development · 2024Article
- Article
- Review
- Human Cytomegalovirus Compromises Development of Cerebral Organoids.Journal of virology · 2019Article
- Astrocyte-enriched feeder layers from cryopreserved cells support differentiation of spontaneously active networks of human iPSC-derived neurons.Journal of neuroscience methods · 2018Article
- Reproducible and efficient generation of functionally active neurons from human hiPSCs for preclinical disease modeling.Stem cell research · 2018Article
- Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research.Journal of visualized experiments : JoVE · 2016Article
- Model systems for studying cellular mechanisms of SCN1A-related epilepsy.Journal of neurophysiology · 2016Review
- A novel, long-lived, and highly engraftable immunodeficient mouse model of mucopolysaccharidosis type I.Molecular therapy. Methods & clinical development · 2015Article
- Identification of fragile X syndrome specific molecular markers in human fibroblasts: a useful model to test the efficacy of therapeutic drugs.Human mutation · 2014Article
- Repression of SIRT1 promotes the differentiation of mouse induced pluripotent stem cells into neural stem cells.Cellular and molecular neurobiology · 2014Article
- Functional differentiation of midbrain neurons from human cord blood-derived induced pluripotent stem cells.Stem cell research & therapy · 2014Article
- Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix.World journal of stem cells · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Robust strategies for developing patient-specific, human, induced pluripotent stem cell (iPSC)-based therapies of the brain require an ability to derive large numbers of highly defined neural cells. Recent progress in iPSC culture techniques includes partial-to-complete elimination of feeder layers, use of defined media, and single-cell passaging. However, these techniques still require embryoid body formation or coculture for differentiation into neural stem cells (NSCs). In addition, none of the published methodologies has employed all of the advances in a single culture system. Here we describe a reliable method for long-term, single-cell passaging of PSCs using a feeder-free, defined culture system that produces confluent, adherent PSCs that can be differentiated into NSCs. To provide a basis for robust quality control, we have devised a system of cellular nomenclature that describes an accurate genotype and phenotype of the cells at specific stages in the process. We demonstrate that this protocol allows for the efficient, large-scale, cGMP-compliant production of transplantable NSCs from all lines tested. We also show that NSCs generated from iPSCs produced with the process described are capable of forming both glia defined by their expression of S100β and neurons that fire repetitive action potentials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.