ArticleJournal of visualized experiments : JoVE2013
Differentiation of newborn mouse skin derived stem cells into germ-like cells in vitro.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded.Cells · 2020Review
- Retinoic acid enhances germ cell differentiation of mouse skin-derived stem cells.Journal of ovarian research · 2018Article
- Involvement of blood mononuclear cells in the infertility, age-associated diseases and cancer treatment.World journal of stem cells · 2016Review
- Skin-derived stem cells as a source of primordial germ cell- and oocyte-like cells.Cell death & disease · 2016Review
- Novel methods of treating ovarian infertility in older and POF women, testicular infertility, and other human functional diseases.Reproductive biology and endocrinology : RB&E · 2015Article
- Differentiation of human umbilical cord matrix-derived mesenchymal stem cells into germ-like cells.Avicenna journal of medical biotechnology · 2014Article
- Ovarian adult stem cells: hope or pitfall?Journal of ovarian research · 2014Review
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
Studying germ cell formation and differentiation has traditionally been very difficult due to low cell numbers and their location deep within developing embryos. The availability of a "closed" in vitro based system could prove invaluable for our understanding of gametogenesis. The formation of oocyte-like cells (OLCs) from somatic stem cells, isolated from newborn mouse skin, has been demonstrated and can be visualized in this video protocol. The resulting OLCs express various markers consistent with oocytes such as Oct4 , Vasa , Bmp15, and Scp3. However, they remain unable to undergo maturation or fertilization due to a failure to complete meiosis. This protocol will provide a system that is useful for studying the early stage formation and differentiation of germ cells into more mature gametes. During early differentiation the number of cells expressing Oct4 (potential germ-like cells) reaches ~5%, however currently the formation of OLCs remains relatively inefficient. The protocol is relatively straight forward though special care should be taken to ensure the starting cell population is healthy and at an early passage.
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Registered trials
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