ArticleJournal of industrial microbiology & biotechnology2013
Selective enrichment and production of highly urease active bacteria by non-sterile (open) chemostat culture.
Article in Journal of industrial microbiology & biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 84 citations in OpenAlex.
- Restaurant wastewater as a sustainable medium for ureolytic bacteria in biocementation.World journal of microbiology & biotechnology · 2026Article
- Enhanced MICP for Soil Improvement and Heavy Metal Remediation: Insights from Landfill Leachate-Derived Ureolytic Bacterial Consortium.Microorganisms · 2025Article
- Experimental Study on High-Temperature Damage Repair of Concrete by Soybean Urease Induced Carbonate Precipitation.Materials (Basel, Switzerland) · 2022Article
- Urease production using corn steep liquor as a low-cost nutrient source by Sporosarcina pasteurii: biocementation and process optimization via artificial intelligence approaches.Environmental science and pollution research international · 2022Article
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- Research status and development of microbial induced calcium carbonate mineralization technology.PloS one · 2022Article
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- Article
- Influence ofHeliyon · 2019Article
- Environmental safety and biosafety in construction biotechnology.World journal of microbiology & biotechnology · 2019Review
- Urease-aided calcium carbonate mineralization for engineering applications: A review.Journal of advanced research · 2018Review
- Bioprospecting of Ureolytic Bacteria From Laguna Salada for Biomineralization Applications.Frontiers in bioengineering and biotechnology · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In general, bioprocesses can be subdivided into naturally occurring processes, not requiring sterility (e.g., beer brewing, wine making, lactic acid fermentation, or biogas digestion) and other processes (e.g., the production of enzymes and antibiotics) that typically require a high level of sterility to avoid contaminant microbes overgrowing the production strain. The current paper describes the sustainable, non-sterile production of an industrial enzyme using activated sludge as inoculum. By using selective conditions (high pH, high ammonia concentration, and presence of urea) for the target bacterium, highly active ureolytic bacteria, physiologically resembling Sporosarcina pasteurii were reproducibly enriched and then continuously produced via chemostat operation of the bioreactor. When using a pH of 10 and about 0.2 M urea in a yeast extract-based medium, ureolytic bacteria developed under aerobic chemostat operation at hydraulic retention times of about 10 h with urease levels of about 60 μmol min⁻¹ ml⁻¹ culture. For cost minimization at an industrial scale the costly protein-rich yeast extract medium could be replaced by commercial milk powder or by lysed activated sludge. Glutamate, molasses, or glucose-based media did not result in the enrichment of ureolytic bacteria by the chemostat. The concentration of intracellular urease was sufficiently high such that the produced raw effluent from the reactor could be used directly for biocementation in the field.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.