ArticleNucleic acids research2013
Synthetic tolerance: three noncoding small RNAs, DsrA, ArcZ and RprA, acting supra-additively against acid stress.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 98 citations in OpenAlex.
- From Environmental Persistence to Host Adaptation: Differential Curli Regulation inMicroorganisms · 2026Review
- Advances in stress-tolerance elements for microbial cell factories.Synthetic and systems biotechnology · 2024Review
- Engineering quorum sensing-based genetic circuits enhances growth and productivity robustness of industrial E. coli at low pH.Microbial cell factories · 2024Article
- Achieving robust synthetic tolerance in industrialSynthetic and systems biotechnology · 2024Article
- CRISPR-dCas13a system for programmable small RNAs and polycistronic mRNA repression in bacteria.Nucleic acids research · 2024Article
- Understanding the Streptomyces albulus response to low-pH stress at the interface of physiology and transcriptomics.Applied microbiology and biotechnology · 2023Article
- The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023Article
- Revealing novel synergistic defense and acid tolerant performance of Escherichia coli in response to organic acid stimulation.Applied microbiology and biotechnology · 2022Article
- PhoP induces RyjB expression under acid stress in Escherichia coli.Journal of biochemistry · 2022Article
- How global RNA-binding proteins coordinate the behaviour of RNA regulons: An information approach.Computational and structural biotechnology journal · 2022Article
- The challenges and prospects of Escherichia coli as an organic acid production host under acid stress.Applied microbiology and biotechnology · 2021Review
- A Shift to Human Body Temperature (37°C) Rapidly Reprograms Multiple Adaptive Responses in Escherichia coli That Would Facilitate Niche Survival and Colonization.Journal of bacteriology · 2021Article
- Engineering of the Small Noncoding RNA (sRNA) DsrA Together with the sRNA Chaperone Hfq Enhances the Acid Tolerance of Escherichia coli.Applied and environmental microbiology · 2021Article
- Pantoea ananatis carotenoid production confers toxoflavin tolerance and is regulated by Hfq-controlled quorum sensing.MicrobiologyOpen · 2021Article
- Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The Small RNA sr8384 Is a Crucial Regulator of Cell Growth in Solventogenic Clostridia.Applied and environmental microbiology · 2020Article
- Multiple Small RNAs Interact to Co-regulate Ethanol Tolerance inFrontiers in bioengineering and biotechnology · 2020Article
- Systemic understanding of Lactococcus lactis response to acid stress using transcriptomics approaches.Journal of industrial microbiology & biotechnology · 2019Article
- On a stake-out:Non-coding RNA research · 2019Review
- Enhanced acid-stress tolerance in Lactococcus lactis NZ9000 by overexpression of ABC transporters.Microbial cell factories · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic acid tolerance, especially during active cell growth, is a desirable phenotype for many biotechnological applications. Natively, acid resistance in Escherichia coli is largely a stationary-phase phenotype attributable to mechanisms mostly under the control of the stationary-phase sigma factor RpoS. We show that simultaneous overexpression of noncoding small RNAs (sRNAs), DsrA, RprA and ArcZ, which are translational RpoS activators, increased acid tolerance (based on a low-pH survival assay) supra-additively up to 8500-fold during active cell growth, and provided protection against carboxylic acid and oxidative stress. Overexpression of rpoS without its regulatory 5'-UTR resulted in inferior acid tolerance. The supra-additive effect of overexpressing the three sRNAs results from the impact their expression has on RpoS-protein levels, and the beneficial perturbation of the interconnected RpoS and H-NS networks, thus leading to superior tolerance during active growth. Unlike the overexpression of proteins, overexpression of sRNAs imposes hardly any metabolic burden on cells, and constitutes a more effective strain engineering strategy.
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