Evidence map›Paper›PMID 23892194›Full record

ArticleBiophysical chemistry

The pH dependence of staphylococcal nuclease stability is incompatible with a three-state denaturation model.

Daniel Spencer, García-Moreno E Bertrand, Wesley E Stites

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical chemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Daniel SpencerDepartment of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR 72701, USA.
García-Moreno E Bertrand
Wesley E Stites
University of Arkansas at Fayetteville · USJohns Hopkins University · US

Funding

Structure based drug discoveryP20RR015569 · NCRR · UNIVERSITY OF ARKANSAS AT FAYETTEVILLE · PI MILLETT, FRANCIS S · 2000 to 2009
$21.4M
Center for Protein Structure and FunctionP30GM103450 · NIGMS · UNIVERSITY OF ARKANSAS AT FAYETTEVILLE · PI MILLETT, FRANCIS S · 2012 to 2014
$3.2M
Electrostatic effects of the native state ensembleR01GM073838 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI GARCIA-MORENO, BERTRAND · 2009 to 2012
$1.4M
NCRR NIH HHS P20 RR015569NCRR NIH HHS P20 RR15569NIGMS NIH HHS GM073838NIGMS NIH HHS P30 GM103450NIGMS NIH HHS R01 GM073838
6 · The paper itself

Abstract

Six single substitution mutations, V66F, V66G, V66N, V66Q, V66S, V66T, and V66Y, were made in the background of a highly stable triple mutant (P117G, H124L, and S128A) of staphylococcal nuclease. The thermodynamic stabilities of wild type staphylococcal nuclease, of the stable triple mutant and of its six variants were determined by guanidine hydrochloride denaturation in thirteen different buffers spanning the pH range 4.5 to 10.2. Within experimental error the values of [Formula: see text] and mGuHCl for the various proteins measured over this wide range of pH maintain a constant offset from one another, tracing a series of approximately parallel curves. This data offers an independent means of determining the error of stabilities and slopes determined by guanidine hydrochloride denaturations and shows that previous error estimates are accurate. More importantly, this behavior cannot be reconciled with a three-state denaturation model for staphylococcal nuclease. The large variations in mGuHCl observed in these mutants must therefore arise from other causes.

Indexed as

Amino Acid SubstitutionGuanidineHydrogen-Ion ConcentrationMicrococcal NucleaseProtein DenaturationProtein StabilityStaphylococcusThermodynamicsGuanidineMicrococcal NucleaseElectrostatic effectEquilibrium denaturationEquilibrium intermediateProtein foldingProtein stabilitySlope

Identifiers

PMID23892194
PMCPMC3770747
OpenAlexW2087524381

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.